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首页> 外文期刊>Biochemical and Biophysical Research Communications >Myosin XI localizes at the mitotic spindle and along the cell plate during plant cell division in Physcomitrella patens
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Myosin XI localizes at the mitotic spindle and along the cell plate during plant cell division in Physcomitrella patens

机译：Myosin Xi在Physcomitrella的植物细胞分裂期间定位在有丝分裂主轴和沿着细胞板上

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摘要

Cell division is a fundamental biological process that has been extensively investigated in different systems. Similar to most eukaryotic cells, plant cells assemble a mitotic spindle to separate replicated chromosomes. In contrast, to complete cell division, plant cells assemble a phragmoplast, which is composed of aligned microtubules and actin filaments. This structure helps transport vesicles containing new cell wall material, which then fuse to form the cell plate; the cell plate will expand to create the new dividing cell wall. Because vesicles are known to be transported by myosin motors during interphase, we hypothesized this could also be the case during cell division and we investigated the localization of the plant homologue of myosin V - myosin XI, in cell division. In this work, we used the protonemal cells of the mossPhyscomitrella patensas a model, because of its simple cellular morphology and ease to generate transgenic cell lines expressing fluorescent tagged proteins. Using a fluorescent protein fusion of myosin XI, we found that, during mitosis, this molecule appears to associate with the kinetochores immediately after nuclear envelope breakdown. Following metaphase, myosin XI stays associated with the spindle's midzone during the rest of mitosis, and when the phragmoplast is formed, it concentrates at the cell plate. Using an actin polymerization inhibitor, latrunculin B, we found that the association of myosin XI with the mitotic spindle and the phragmoplast are only partially dependent on the presence of filamentous actin. We also showed that myosin XI on the spindle partially overlaps with a v-SNARE vesicle marker but is not co-localized with the endoplasmic reticulum and a RabA vesicle marker. These observations suggest an actin-dependent and an actin-independent behavior of myosin XI during cell division, and provide novel insights to our understanding of the function of myosin XI during plant cell division.
机译：细胞分裂是在不同系统中广泛调查的基本生物过程。与大多数真核细胞类似，植物细胞组装有丝分子纺锤体以分离复制的染色体。相反，对于完全细胞划分，植物细胞组装脓血血糖，其由对齐的微管和肌动蛋白细丝组成。该结构有助于将含有新的细胞壁材料的囊泡运输，然后熔化以形成细胞板;电池板将扩展以创建新的分割细胞壁。因为已知囊泡在间作期间被肌蛋白电动机运输，所以我们假设其在细胞分裂期间也是如此，我们研究了细胞分裂中肌蛋白v - myosin xi的植物同源物的定位。在这项工作中，我们使用了Mossphyscomitrella Patensas的原粒体细胞是一种模型，因为其简单的细胞形态和易于产生表达荧光标记蛋白的转基因细胞系。我们发现，在有丝分裂期间，在核心包络击穿后，该分子似乎将该分子与Kinetochores联系起来。继术后，肌球蛋白Xi在剩下的菌丝病症期间与主轴的中间座保持联系，并且当形成脊髓膜时，它将其集中在细胞板上。使用肌动蛋白聚合抑制剂Latrunculin B，我们发现肌球蛋白Xi与有丝分裂主轴和脓脊椎网的关联仅部分依赖于丝状肌动蛋白的存在。我们还表明，脊柱肌肉Xi部分与V-Snare囊泡标记部分重叠，但不与内质网和Raba囊泡标记共同定位。这些观察结果表明，在细胞划分期间肌动蛋白依赖于肌动蛋白XI的肌动蛋白Xi，并为我们对植物细胞划分期间的肌球蛋白XI功能的理解提供了新的见解。

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