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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >Label-free and ultrasensitive electrochemical biosensor for the detection of EBV-related DNA based on AgDNCs@DNA/AgNCs nanocomposites and lambda exonuclease-assisted target recycling
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Label-free and ultrasensitive electrochemical biosensor for the detection of EBV-related DNA based on AgDNCs@DNA/AgNCs nanocomposites and lambda exonuclease-assisted target recycling

机译：无抗敏和超细电化学生物传感器，用于检测基于AGDNCS @ DNA / AGNCS纳米复合材料和Lambda Exonuclease辅助目标回收的EBV相关的DNA

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摘要

A label-free and efficient electrochemical biosensor was developed for the ultrasensitive detection of EBV-related DNA by combing AgDNCs@DNA/AgNCs nanocomposites with noncanonical lambda exonuclease (lambda exo)-assisted target recycling (LNTR). The conjugates of AgDNCs, DNA/AgNCs and probe DNA (pDNA-AgDNCs@DNA/AgNCs conjugates) worked as not only ideal nanocarriers but also efficient electrochemical tags. LNTR didn't require phosphorylated substrates and could be triggered specifically by target DNA, leading to the recycling use of target DNA and the liberation of plentiful linker probes (LP). Subsequently, the LP hybridized with the capture probes on the electrode and then bond to pDNA-AgDNCs@DNA/AgNCs conjugates, generating a sensitive electric signal directly. What's more, the signal amplification effects of DNA/AgNCs and LNTR were investigated. Under the optimal conditions, the proposed method exhibited a wide linear range of 1 fM to 1 nM and the detection limit down to 0.38 fM. In addition, the developed biosensing method exhibited excellent specificity and was successfully applied to detect target DNA in complex biological matrix. The proposed biosensor without extra bio-labels may provide a promising platform in bioanalysis and biochemical research.
机译：通过梳理AgDNCS @ DNA / Agncs纳米复合材料与非Canonical Lambda Exonuclease（LNBDA EXO）靶回收（LNTR）来开发出无抗体和有效的电化学生物传感器进行无抗敏检测。 AGDNC，DNA / AGNC和探针DNA的缀合物（PDNA-AGDNCS @ DNA / AgNCS缀合物）不仅适用于理想的纳米载体，而且还具有高效的电化学标签。 LNTR不需要磷酸化底物，并且可以通过靶DNA具体触发，导致靶DNA的再循环使用和充分的接头探针（LP）的释放。随后，LP与电极上的捕获探针杂交，然后与PDNA-AGDNCS @ DNA / AGNCS缀合物键合，直接产生敏感的电信号。更重要的是，研究了DNA / AGNC和LNTR的信号放大效应。在最佳条件下，所提出的方法表现出1 Fm至1nm的宽线性范围，并且检测限度降至0.38 fm。此外，开发的生物传感方法表现出优异的特异性，并成功地应用于复杂生物基质中的靶DNA。没有额外生物标签的拟议生物传感器可以提供有希望的生物分析和生物化学研究的平台。

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