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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >Tailored monoclonal antibody as recognition probe of immunosensor for ultrasensitive detection of silk fibroin and use in the study of archaeological samples
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Tailored monoclonal antibody as recognition probe of immunosensor for ultrasensitive detection of silk fibroin and use in the study of archaeological samples

机译：定制单克隆抗体作为免疫传感器的识别探针，用于防丝素蛋白的超细素和用于考古样品的研究

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The ultrasensitive detection of fibroin in unearthed silk relics has great significance for investigating the origin and transmission of silk. In this study, an anti-fibroin monoclonal antibody was successfully prepared through animal immunization. Next, a label-free electrochemical immunosensor was fabricated using layer-by-layer self-assembly technology, and an indirect enzyme-linked immunosorbent assay (ELISA) was proposed. The two methods exhibited excellent sensitivity and specificity in the detection of silk fibroin, while the immunosensor showed a wider quantitative detection range (0.1-100 ng mL(-1)) and a lower detection limit (0.051 ng mL(-1)) than ELISA (10-100 ng mL(-1) and 8.71 ng mL(-1)). Furthermore, the performance of the immunosensor was superior in archaeological sample detection. Taking advantage of the well-prepared monoclonal antibody, the two proposed immunological assays demonstrate tremendous potential for the ultrasensitive detection of silk fibroin, which can make great contributions to exploring the origin and transmission routes of ancient silks.
机译：在出土的丝绸遗物中的肌蛋白的超敏检测具有重要意义，对丝绸的起源和传输具有重要意义。在该研究中，通过动物免疫成功制备抗纤维素单克隆抗体。接下来，使用逐层自组装技术制造无标签电化学免疫传感器，提出了间接酶联免疫吸附测定（ELISA）。两种方法在丝素蛋白的检测中表现出优异的敏感性和特异性，而免疫传感器显示出更宽的定量检测范围（0.1-100ng ml（-1））和较低的检测极限（0.051 ng ml（-1）） ELISA（10-100ng ml（-1）和8.71 ng ml（-1））。此外，免疫传感器的性能在考古样本检测中优异。利用良好制备的单克隆抗体，这两个提出的免疫测定结果表明了丝素蛋白的超细敏感检测的巨大潜力，这可以为探索古代丝绸的起源和传输路线提供巨大贡献。

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