...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >A novel electrochemical biosensor for ultrasensitive detection of serum total bile acids based on enzymatic reaction combined with the double oxidation circular amplification strategy
【24h】

A novel electrochemical biosensor for ultrasensitive detection of serum total bile acids based on enzymatic reaction combined with the double oxidation circular amplification strategy

机译：一种新型电化学生物传感器，用于基于酶法反应的血清总胆汁酸的超敏感检测与双氧化圆形扩增策略联合

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Serum total bile acids (TBA) level is used as a sensitive and reliable index for hepatobiliary diseases in clinics. Herein, a novel electrochemical biosensor was fabricated using enzymatic reaction coupling with the double oxidation circular amplification strategy for the detection of human serum TBA. With the catalysis of 3α-hydroxysteroid dehydrogenase (3α-HSD), 3α-bile acids reacted specifically with nicotinamide adenine dinucleotide (NAD+). And then, the reduced nicotinamide adenine dinucleotide (NADH) was produced. After that, the NADH reacted with the electron mediator of tris(2,2'-bipyridine) ruthenium(Ⅲ) (Ru(bpy)33+), which was then transformed to Ru(bpy)32+. Ultimately, Ru(bpy)32+was further oxidized to Ru(bpy)33+under a certain voltage, which was detected by the chronoamperometry assay. The detection was performed using a disposable unmodified screen-printed carbon electrode (SPCE) without sample preparation. The proposed biosensor showed high sensitivity and accuracy with the linear range from 5.0 to 150.0 pmol/L in 106-fold dilution serum. The established method had a good correlation with the enzymatic cycling method (r?=?0.9372,P?
机译：血清总胆汁酸（TBA）水平被用作诊所肝胆疾病的敏感性和可靠指标。在此，使用酶促反应偶联与双氧化圆形扩增策略进行检测的双氧化圆形扩增策略制备一种新型电化学生物传感器。随着3α-羟类脱氢酶（3α-HSD）的催化，3α-胆汁酸与烟酰胺腺嘌呤二核苷酸（NAD +）有特异性反应。然后，制备烟酰胺腺嘌呤腺嘌呤二核苷酸（NADH）。之后，NADH与TRIS（2,2'-硼吡啶）钌（Ⅲ）（Ru（BPY）33+）的电子介体反应，然后将其转化为Ru（BPY）32+。最终，Ru（BPY）32 +在一定电压下进一步被氧化成Ru（BPY）33 +，该电压由计时法检测。使用一次性未改性的丝网印刷的碳电极（SPCE）进行检测而没有样品制备。该拟议的生物传感器显示出高灵敏度和精度，线性范围为5.0至150.0pmol / L在106倍稀释血清中。该已建立的方法与酶常用的酶循环方法（R？= 0.9372，p≤0.9372，p≤0.<〜0.001，n≤x≤72）良好。电化学生物传感器简单，超声，无样本预处理，显示出临床样品中血清TBA的护理点测试（POCT）的巨大潜力。此外，生物传感器具有成本效益，少量样品，特别适用于血液收集困难的人，例如婴儿，儿童和一些小动物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号