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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >A wavelength-modulated localized surface plasmon resonance (LSPR) optical fiber sensor for sensitive detection of mercury(II) ion by gold nanoparticles-DNA conjugates
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A wavelength-modulated localized surface plasmon resonance (LSPR) optical fiber sensor for sensitive detection of mercury(II) ion by gold nanoparticles-DNA conjugates

机译：用于通过金纳米颗粒-DNA缀合物敏感汞（II）离子的敏感检测的波长调制局部表面等离子体谐振（LSPR）光纤传感器

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摘要

The study presented herein investigated an easy preparation, high performance, wavelength-modulated LSPR optical fiber chemosensor coated by gold nanospheres(AuNS) for Hg2+ detection based on thymine-Hg2+-thymine base pair mismatches and the coupled plasmonic resonance effect.Utilizing electrostatic self-assembly method, the high density and dispersivity monolayer AuNS coated LSPR fiber sensor had the near field refractive index sensitivity up to 2016 nm/RIU. The single-strand probe DNA served as a binding element for free AuNS labelled-target DNA conjugates was attached to the monolayer AuNS by Au-S bond. In the present of Hg2+, the coupled plasmonic resonance band between monolayer AuNS and free AuNS was produced by thymine-Hg2+-thymine structure and leaded to red-shift of LSPR peak. Under the optimal conditions, the enlarged red-shift in peak of LSPR spectroscopy was linearly with the concentration of Hg2+ in the range from 1.0 x 10(-9) to 5.0 x 10(-8) M with the coefficient of 0.976. The limit of detection was 0.7 nM(S/N = 3). The specificity of the sensor was proved high by evaluating the response to other heavy metal ions. The proposed fiber sensor provided a label-free, miniature, low-cost approach for the Hg2+ detection and had potential in real environmental evaluations.
机译：本文提出的研究研究了基于胸腺嘧啶-HG2 + -Thymine基对错配和耦合等离子体共振效应的HG2 +检测涂覆的易于制备，高性能，高性能，高性能，高性能，具有用于HG2 +检测的HG2 +检测。装配方法，高密度和分散性单层AUNS涂层LSPR光纤传感器的近似距离折射率敏感性高达2016 NM / RIU。用Au-S键将单链探针DNA用作可自由αSuns标记的靶DNA缀合物的末端α缀合物。在HG2 +的目的中，单层肛门和自由肛门之间的耦合等离子体共振带由胸腺嘧啶-HG2 + - + - β-β-α-基于LSPR峰的红色偏移产生。在最佳条件下，LSPR光谱峰的峰值的扩大红转与Hg2 +的浓度线性，其范围为1.0×10（-9）至5.0×10（-8）m，系数为0.976。检测限为0.7nm（s / n = 3）。通过评价对其他重金属离子的响应来证明传感器的特异性。所提出的纤维传感器为HG2 +检测提供了无标记，微型，低成本的方法，并具有实际环境评估中的潜力。

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