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首页> 外文期刊>BioMed research international >Isolation, Screening, and Identification of Cellulolytic Bacteria from Natural Reserves in the Subtropical Region of China and Optimization of Cellulase Production by Paenibacillus terrae ME27-1
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Isolation, Screening, and Identification of Cellulolytic Bacteria from Natural Reserves in the Subtropical Region of China and Optimization of Cellulase Production by Paenibacillus terrae ME27-1

机译：来自中国亚热带地区自然保护区的纤维素溶解细菌的分离，筛选和鉴定及Paenibacillus Terrae Me27-1的纤维素酶生产优化

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From different natural reserves in the subtropical region of China, a total of 245 aerobic bacterial strains were isolated on agar plates containing sugarcane bagasse pulp as the sole carbon source. Of the 245 strains, 22 showed hydrolyzing zones on agar plates containing carboxymethyl cellulose after Congo-red staining. Molecular identification showed that the 22 strains belonged to 10 different genera, with the Burkholderia genus exhibiting the highest strain diversity and accounting for 36.36% of all the 22 strains. Three isolates among the 22 strains showed higher carboxymethyl cellulase (CMCase) activity, and isolate ME27-1 exhibited the highest CMCase activity in liquid culture. The strain ME27-1 was identified as Paenibacillus terrae on the basis of 16S rRNA gene sequence analysis as well as physiological and biochemical properties. The optimum pH and temperature for CMCase activity produced by the strain ME27-1 were 5.5 and 50°C, respectively, and the enzyme was stable at a wide pH range of 5.0-9.5. A 12-fold improvement in the CMCase activity (2.08 U/mL) of ME27-1 was obtained under optimal conditions for CMCase production. Thus, this study provided further information about the diversity of cellulose-degrading bacteria in the subtropical region of China and found P. terrae ME27-1 to be highly cellulolytic.
机译：从中国亚热带地区的不同自然保护区，总共245个有氧细菌菌株，含有甘蔗甘蔗纸浆作为唯一碳源的琼脂平板。在245株的菌株中，22在刚果 - 红染色后含有羧甲基纤维素的琼脂平板上显示水解区。分子鉴定表明，22个菌株属于10种不同的属，伯克德利菌属属呈现出最高的应变多样性，占所有22株菌株的36.36％。 22株中的三个分离物显示出较高的羧甲基纤维素酶（CMCASE）活性，分离物ME27-1在液体培养物中表现出最高的CMCASE活性。基于16S rRNA基因序列分析以及生理学和生化特性，菌株ME27-1被鉴定为Paenibacillus Terrae。由菌株ME27-1产生的CMCase活性的最佳pH和温度分别为5.5和50℃，酶在5.0-9.5的宽pH范围内稳定。在CMCase生产的最佳条件下获得ME27-1的CMCase活性（2.08U / mL）的12倍改善。因此，本研究提供了有关中国亚热带地区纤维素降解细菌的多样性的进一步信息，并发现P. Terrae Me27-1是高度纤维化的。

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