...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BioMed research international >Diversity and Enzymatic Profiling of Halotolerant Micromycetes from Sebkha El Melah, a Saharan Salt Flat in Southern Tunisia
【24h】

Diversity and Enzymatic Profiling of Halotolerant Micromycetes from Sebkha El Melah, a Saharan Salt Flat in Southern Tunisia

机译：南突尼斯南部撒哈拉盐平面撒哈拉盐（Sebkha El Melah）的多元化和酶促谱分析

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Twenty-one moderately halotolerant fungi have been isolated from sample ashes collected from Sebkha El Melah, a Saharan salt flat located in southern Tunisia. Based on morphology and sequence inference from the internal transcribed spacer regions, 28S rRNA gene and other specific genes such as beta-tubulin, actin, calmodulin, and glyceraldehyde-3-phosphate dehydrogenase, the isolates were found to be distributed over 15 taxa belonging to 6 genera of Ascomycetes: Cladosporium (n = 3), Alternaria (n = 4), Aspergillus (n = 3), Penicillium (n = 5), Ulocladium (n = 2), and Engyodontium (n = 2). Their tolerance to different concentrations of salt in solid and liquid media was examined. Excepting Cladosporium cladosporioides JA18, all isolates were considered as alkali-halotolerant since they were able to grow in media containing 10% of salt with an initial pH 10. All isolates were resistant to oxidative stresses and low temperature whereas 5 strains belonging to Alternaria, Ulocladium, and Aspergillus genera were able to grow at 45° C. The screening of fungal strains for sets of enzyme production, namely, cellulase (CMCase), amylase, protease, lipase, and laccase, in presence of 10% NaCl, showed a variety of extracellular hydrolytic and oxidative profiles. Protease was the most abundant enzyme produced whereas laccase producers were members of the genus Cladosporium.
机译：二十一体中间黑凝珠特真菌已从Sebkha El Melah收集的灰烬中的样品中分离出来，位于突尼斯南部的撒哈拉盐平。基于形态和序列推断从内部转录的间隔区，28s rRNA基因和其他特定基因如β-微管蛋白，肌动蛋白，钙调蛋白和甘油醛-3-磷酸脱氢酶，分离株分布在15个征收6属ascomycetes：囊孢子孢子（n = 3），areralaria（n = 4），曲霉菌（n = 3），高分子（n = 5），ulocladium（n = 2），和engyodontium（n = 2）。检查它们对不同浓度的固体和液体培养基中的不同浓度的耐受性。除囊孢子孢子虫JA18外，所有分离株被认为是碱 - 黑酰凝集剂，因为它们能够在含有初始pH10的含有10％盐的培养基中生长。所有分离株都抗氧化应激和低温，而属于aleraria的5个菌株，uLocladium ，并且曲霉属能够在45℃下生长。酶生产的酶生产，即纤维素酶（CMCase），淀粉酶，蛋白酶，脂肪酶和漆酶存在于10％NaCl的情况下，筛选真菌菌株，显示出各种各样的细胞外水解和氧化谱。蛋白酶是产生的最丰富的酶，而漆酶生产商是族孢子孢子属的成员。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号