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首页> 外文期刊>BioMed research international >Toxicity of Graphene Shells, Graphene Oxide, and Graphene Oxide Paper Evaluated with Escherichia coli Biotests
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Toxicity of Graphene Shells, Graphene Oxide, and Graphene Oxide Paper Evaluated with Escherichia coli Biotests

机译：用大肠杆菌获得石墨烯壳，石墨烯氧化物和石墨烯氧化物纸的毒性

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摘要

The plate-like graphene shells (GS) produced by an original methane pyrolysis method and their derivatives graphene oxide (GO) and graphene oxide paper (GO-P) were evaluated with luminescent Escherichia coli biotests and additional bacterial-based assays which together revealed the graphene-family nanomaterials' toxicity and bioactivity mechanisms. Bioluminescence inhibition assay, fluorescent two-component staining to evaluate cell membrane permeability, and atomic force microscopy data showed GO expressed bioactivity in aqueous suspension, whereas GS suspensions and the GO-P surface were assessed as nontoxic materials. The mechanism of toxicity of GO was shown not to be associated with oxidative stress in the targeted soxSv.lux and katGv.lux reporter cells; also, GO did not lead to significant mechanical disruption of treated bacteria with the release of intracellular DNA contents into the environment. Ihe well-coordinated time- and dose-dependent surface charge neutralization and transport and energetic disorders in the Escherichia coli cells suggest direct membrane interaction, internalization, and perturbation (i.e., "membrane stress") as a clue to graphene oxide's mechanism of toxicity.
机译：用原始甲烷热解法产生的板状石墨烯壳（GS）及其衍生物石墨烯氧化物（GO）和石墨烯氧化物纸（GO-P）进行评价，并用发光的大肠杆菌生物酶和另外的基于细菌的测定来评价一起揭示的石墨烯家族纳米材料的毒性与生物活性机制。生物发光抑制测定，荧光双组分染色以评估细胞膜渗透性，并且原子力显微镜数据显示在水性悬浮液中表达生物活性，而GS悬浮液和Go-P表面被评估为无毒材料。 Go的毒性机制显示不与靶向SOXSv.Lux和Katgv.Lux报告细胞中的氧化应激相关;此外，随着细胞内DNA含量释放到环境中，GO没有导致治疗细菌的重大机械破坏。大肠杆菌细胞中，IHE协调的时间和剂量依赖性表面电荷中和和运输和能量疾病提示直接膜相互作用，内化和扰动（即“膜应力”）作为石墨烯氧化物机制的线索。

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