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首页> 外文期刊>Behavioural Brain Research: An International Journal >Use of a force-sensing automated open field apparatus in a longitudinal study of multiple behavioral deficits in CAG140 Huntington's disease model mice
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Use of a force-sensing automated open field apparatus in a longitudinal study of multiple behavioral deficits in CAG140 Huntington's disease model mice

机译：在CAG140亨廷顿疾病模型小鼠中使用一种力传感自动开放式设备在纵向研究中的纵向研究中的多种行为缺陷

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Behavioral testing of mouse models of Huntington's disease (HD) is a key component of preclinical assessment for potential pharmacological intervention. An open field with a force plate floor was used to quantify numerous spontaneous behaviors in a slowly progressing model of HD. CAG140 (+/+,+/-, -/-) male and female mice were compared in a longitudinal study from 6 to 65 weeks of age. Distance traveled, wall rears, wall rear duration, number of low mobility bouts, in-place movements, number of high velocity runs, and gait parameters (stride rate, stride length, and velocity) were extracted from the ground reaction forces recorded in 20-min actometer sessions. Beginning at 11 weeks, HD mice (both +/- and +/+) were consistently hypoactive throughout testing. Robust hypoactivity at 39 weeks of age was not accompanied by gait disturbances. By 52 and 65 weeks of age the duration of wall rears increased and in-place tremor-like movements emerged at 65 weeks of age in the +/+, but not in the +/-HD mice. Taken together, these results suggest that hypoactivity preceding frank motor dysfunction is a characteristic of CAG140 mice that may correspond to low motivation to move seen clinically in the premanifest/prediagnostic stage in human HD. The results also show that the force plate method provides a means for tracking the progression of behavioral dysfunction in HD mice beyond the stage when locomotion is lost while enabling quantification of tremor-like and similar in-place behaviors without a change in instrumentation. Use of force plate actometry also minimizes testing-induced enrichment effects when batteries of different tests are carried out longitudinally. (C) 2015 Elsevier B.V. All rights reserved.
机译：亨廷顿疾病（HD）小鼠模型的行为测试是潜在药理学干预的临床前评估的关键组成部分。具有力板地板的开放场用于在缓慢进展的HD模型中量化许多自发性行为。将CAG140（+ / +，+ / - ， - ）男性和雌性小鼠在6至65周龄的纵向研究中进行了比较。从20个记录的地面反作用力提取出来的距离行驶，壁后部，壁后持续时间，低移动性偏移，就地运动，高速运动的数量，高速速度，以及步态参数（步幅，步幅长度和速度） -min演奏计会话。从11周开始，HD小鼠（+/-和+ / +）在整个测试过程中都是持续的。 39周龄的强大的次次乳房伴随着步态紊乱。在52期和65周龄的年龄上，壁后续的持续时间增加，在+ / +的65周龄，但不在+/-HD小鼠中出现的震颤等运动。这些结果表明，弗兰克电动机功能障碍的脱催化剂是CAG140小鼠的特征，其可能对应于在人HD中的初始/预诊断阶段临床上临床上移动的低动力。结果还表明，当机车丢失时，该力板方法提供了用于跟踪超出阶段之外的HD小鼠的行为功能障碍进展的方法，同时在没有仪器的变化的情况下使震颤和类似的就地行为。当纵向进行不同测试的电池时，使用力板静电测量还会最大限度地减少测试诱导的富集效应。 （c）2015 Elsevier B.v.保留所有权利。

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