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The inhibitory effect of different patterns of low frequency stimulation on neuronal firing following epileptiform activity in rat hippocampal slices

机译：低频刺激不同模式对大鼠海马切片癫痫发生后神经元烧制的抑制作用

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摘要

Low frequency stimulation (LFS) has inhibitory effect on hyperexcitability during epileptic states. However, knowledge is lacking about LFS patterns that can exert an optimal antiepileptic effect. In this study, the effect of different numbers of pulses and current intensities of 1 Hz LFS applied at various time points of epileptiform activity was evaluated in high-K+ model of epileptiform activity (EA). LFS was applied to the Schaffer col laterals, and changes in the excitability of CA1 pyramidal neurons were measured using whole-cell patch-clamp recording. Six hundred and 900 pulses of LFS at two current intensities (equal to and 1.5 times greater than the current intensity sufficient to elicit a 5 mV EPSP) administered at the beginning of EA revealed a stronger LFS inhibitory effect on EA-induced neuronal hyperexcitability when applied at higher pulse number and current intensity. LFS900 (high intensity) significantly hyperpolarized the membrane potential after a high-K+ ACSF washout, reduced the frequency of spontaneous action potentials during EA, and attenuated neuronal firing frequency after high-K+ ACSF washout. Moreover, applying LFS900 (high intensity) before EA induction and 8-10 min after EA initiation could not significantly affect neuronal hyperexcitability, compared to its application at the beginning of EA. This study's findings also offered long-term depression (LTD) as a probable mechanism for LFS' inhibitory role on EA-induced neuronal hyperexcitability. Therefore, the application of LFS (1 Hz) at 900 pulses and greater current intensity at the beginning of EA can exert a strong inhibitory effect on EA-induced neuronal hyperexcitability.
机译：低频刺激（LFS）对癫痫态的过度兴奋性具有抑制作用。然而，知识缺乏关于可以发挥最佳抗癫痫效应的LFS模式。在该研究中，在癫痫型活性（EA）的高k +模型中，评价了在癫痫型活性的各个时间点施加的脉冲和电流强度的影响。将LFS应用于Schaffer Col横向，并且使用全细胞贴片夹具测量Ca1金字塔神经元的兴奋性的变化。在EA开始时施用的两个电流强度的六百和900个LFS的LFS脉冲（相当于足以引出5 mV EPSP的电流强度）在施加时揭示了对EA诱导的神经元过度兴奋性的更强的LFS抑制作用在较高的脉冲数和电流强度处。 LFS900（高强度）显着高k + ACSF冲洗后的膜电位超极化，在高k + acsf冲洗后减少了EA期间的自发动作电位频率。此外，与EA开头的应用相比，在EA诱导前和8-10分钟施用EA诱导和8-10分钟的施用8-10分钟。本研究的调查结果还提供了长期抑郁（LTD）作为LFS抑制对EA诱发的神经元过度尺寸的作用的可能机制。因此，在EA开始时LFS（1Hz）在900个脉冲处的施用和更大的电流强度可以对EA诱导的神经元过度尺寸产生强烈的抑制作用。

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