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首页> 外文期刊>Brain research >Proteomic analysis of brain protein expression levels in NF-kappabeta p50 -/- homozygous knockout mice.
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Proteomic analysis of brain protein expression levels in NF-kappabeta p50 -/- homozygous knockout mice.

机译：NF-Kappabeta P50 - / - 纯合滞后小鼠脑蛋白表达水平的蛋白质组学分析。

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The role of nuclear factor kappa B (NF-kappaB) in oxidative stress, and most recently in pro- and anti-apoptotic-related mechanistic pathways, has well been established. Because of the dual nature of NF-kappaB, the wide range of genes it regulates and the plethora of stimuli that activate it, various studies addressing the functional role of NF-kappaB proteins have resulted in a number of differing findings. The present study examined the effect of a stimulus-free environment on the frontal cortex of mice brain with the p50 subunit of NF-kappaB knocked out p50 (-/-). Homozygous p50 mice knockout (KO) and wild type (WT) were used, and at 7-9 weeks they were sacrificed and various brain regions dissected. We analyzed the levels of oxidation in the frontal cortex of both the p50 (-/-) and WT mice. There was a significant reduction in the levels of protein-bound 4-hydroxynonenal (HNE) [a lipid peroxidation product], 3-nitrotyrosine (3NT), and protein carbonyls in the p50 (-/-) mice when compared to the WT. A proteomic profile analysis identified ATP synthase gamma chain, ubiquinol-cyt-C reductase, heat shock protein 10 (Hsp10), fructose bisphosphate aldolase C, and NADH-ubiquinone oxidoreductase as proteins whose expressions were significantly increased in the p50 (-/-) mice compared to the WT. With the reduction in the levels of oxidative stress and the increase in expression of key proteins in the p50 (-/-) brain, this study suggests that the p50 subunit can potentially be targeted for the development of therapeutic interventions in disorders in which oxidative stress plays a key role.
机译：核因子Kappa B（NF-Kappab）在氧化应激中的作用，以及最近在促凋亡相关的机械途径中，已经建立了很好。由于NF-Kappab的双重性，它调节的广泛基因和激活它的血液刺激，各种研究解决NF-κB蛋白的功能作用导致了许多不同的结果。本研究检测了对小鼠脑正面皮质的无刺激环境与NF-κBP50（ - / - ）的P50亚基对小鼠脑的效果。使用纯合P50小鼠敲除（KO）和野生型（WT），并处死7-9周，并处死各种脑区。我们分析了P50（ - / - ）和WT小鼠的正面皮质中氧化水平。与WT相比，P50（ - / - ）小鼠中，蛋白质结合的4-羟基炔诺（HNE）[脂质过氧化产物]，3-硝基荧光蛋白（3NT）和蛋白质羰基的水平显着降低。蛋白质组学轮廓分析鉴定鉴定ATP合酶γ链，ubiquinol-Cyt-C还原酶，热休克蛋白10（Hsp10），果糖二磷酸醛糖酶C，以及NADH-泛醌氧化还原酶作为P50（ - / - ）显着增加的蛋白质与wt相比的小鼠。随着氧化应激水平的降低和P50（/ - ）脑中的关键蛋白表达的增加，该研究表明P50亚基可能旨在靶向氧化应激的病症中治疗干预措施的发展发挥关键作用。

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