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首页> 外文期刊>Brain research >Electroacupuncture improves cognitive deficits and insulin resistance in an OLETF rat model of Al/D-gal induced aging model via the PI3K/Akt signaling pathway
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Electroacupuncture improves cognitive deficits and insulin resistance in an OLETF rat model of Al/D-gal induced aging model via the PI3K/Akt signaling pathway

机译：通过PI3K / AKT信号通路，电针在Al / D-Gal诱导的老化模型的OLETF大鼠模型中提高了认知缺陷和胰岛素抗性

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摘要

To investigate the effect of electroacupuncture (EA) on cognitive function and insulin resistance (IR) in an Al/D-gal-induced aging model for Alzheimer's disease (AD) using Ostuka Long-Evans Tokushima Fatty (OLETF) rats. The Al/D-gal-OLETF rats for AD were randomly divided into the EA and non-EA groups. Cognitive function was assessed using the Morris water maze (MWM). The morphology of the hippocampal neurons was observed using hematoxylin & eosin (H&E) staining. A beta and total Tau in the hippocampus and cerebrospinal fluid (CSF) were detected using western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). Fasting blood glucose (FPG) was determined using the glucose oxidase method. Plasma fasting insulin (FINS), serum C-peptide (C-P), and CSF insulin were detected using ELISA. The expression of the genes and proteins in the PI3K signaling pathway was detected using quantitative real-time PCR and WB. After EA intervention, the hippocampal A beta and total Tau protein levels, body weight, FPG, FINS, and C-P were significantly decreased. The MWM showed that the percentage of time in the target quadrant of the EA group was elevated in the probe test. The protein levels of p-IRS1, p-IRS2, IDE, and p-GSK3 beta were significantly increased, while p-PI3K-p85a and p-Akt were decreased. In conclusion, EA improves cognitive function and insulin resistance in rat models of AD. The PI3K/Akt signaling pathway is involved in those changes.
机译：探讨电针（EA）对Al / D-Gal诱导的Alzheimer疾病（AS）的认知功能和胰岛素抵抗（IR）的血液抵抗（As）使用Ostuka Long-emans Tokushima脂肪（Oletf）大鼠的影响。 AL / D-GAL-OLETF大鼠随机分为EA和非EA组。使用Morris水迷宫（MWM）评估认知功能。使用苏木精和曙红（H＆E）染色来观察海马神经元的形态。使用蛋白质印迹（WB）和酶联免疫吸附测定（ELISA）检测海马和脑脊液（CSF）中的β和总Tau。使用葡萄糖氧化酶方法测定空腹血糖（FPG）。使用ELISA检测等离子体禁食胰岛素（翅片），血清C肽（C-P）和CSF胰岛素。使用定量实时PCR和Wb检测PI3K信号传导途径中基因和蛋白的表达。在EA干预后，海马β和总Tau蛋白水平，体重，FPG，翅片和C-P显着降低。 MWM显示EA组靶象限中的时间百分比升高在探测试验中。 P-IRS1，P-IRS2，IDE和P-GSK3β的蛋白质水平显着增加，而P-PI3K-P85A和P-AKT被降低。总之，EA提高了广告大鼠模型中的认知功能和胰岛素抗性。 PI3K / AKT信令路径参与这些变化。

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  • 来源
    《Brain research》 |2020年第1期|共9页
  • 作者

    Huang Xinyu; Huang Kangbai; Li Zhaowei; Bai Dongyan; Hao Yan; Wu Qinglong; Yi Wei; Xu Nenggui; Pan Youcan; Zhang Liang;

  • 作者单位

    Guangzhou Univ Chinese Med Clin Med Coll Acupuncture Moxibust &

    Rehabil 232 Waihuan East Rd;

    Guangzhou Univ Chinese Med Clin Med Coll Acupuncture Moxibust &

    Rehabil 232 Waihuan East Rd;

    Guangzhou Univ Chinese Med Coll Sport &

    Hlth Guangzhou 510006 Guangdong Peoples R China;

    Guangzhou Univ Chinese Med Clin Med Coll Acupuncture Moxibust &

    Rehabil 232 Waihuan East Rd;

    Guangzhou Univ Chinese Med Clin Med Coll Acupuncture Moxibust &

    Rehabil 232 Waihuan East Rd;

    Guangzhou Univ Chinese Med Clin Med Coll Acupuncture Moxibust &

    Rehabil 232 Waihuan East Rd;

    Guangzhou Univ Chinese Med Clin Med Coll Acupuncture Moxibust &

    Rehabil 232 Waihuan East Rd;

    Guangzhou Univ Chinese Med Guangzhou 510006 Guangdong Peoples R China;

    Guangzhou Univ Chinese Med Clin Med Coll Acupuncture Moxibust &

    Rehabil 232 Waihuan East Rd;

    Guangzhou Univ Chinese Med Clin Med Coll Acupuncture Moxibust &

    Rehabil 232 Waihuan East Rd;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 神经病学;
  • 关键词

    Acupuncture; Insulin resistance; Alzheimer's disease; beta-Amyloid; Ostuka Long-Evans Tokushima Fatty rats;

    机译：针灸;胰岛素抵抗;阿尔茨海默病;β-淀粉样蛋白;Ostuka Long-evans tokushima脂肪大鼠;

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