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首页> 外文期刊>Bulletin of experimental biology and medicine >Molecular genetic study of species and strain variability in bifidobacteria population in intestinal microflora of breast-fed infants and their mothers.
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Molecular genetic study of species and strain variability in bifidobacteria population in intestinal microflora of breast-fed infants and their mothers.

机译：母乳喂养婴儿及其母亲肠道群体群体群体物种和应变变异性的分子遗传学研究。

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摘要

Qualitative and quantitative composition of enteric bifidoflora was studied in a group of 13 mother-infant pairs. Pure cultures of Bifidobacterium strains were isolated from feces and their species were identified by PCR with species-specific primers or by partial sequencing of 16S rDNA. The strains were compared by REP-PCR. The most incident Bifidobacterium species in mothers were B. longum and B. adolescentis. The infants were mainly colonized by B. bifidum and B. longum. The mother and her baby were colonized by the same Bifidobacterium species in 9 of 13 cases. In 5 (38.5%) of these cases, these pairs of strains were identical by their REP-PCR profiles. These strains belonged to B. longum in one case, B. bifidum in 3 cases, and B. adolescentis in 1 case. Our results support the hypothesis on early colonization of infants with maternal bifidobacterium strains.
机译：在一组13个母婴对中研究了肠道二歧烷的定性和定量组成。 从粪便中分离出双歧杆菌菌株的纯培养物，并通过PCR用物质特异性引物或通过16S rDNA的部分测序鉴定它们的物种。 通过REP-PCR比较菌株。 母亲中最多的离双歧杆菌种类是B. Longum和B. Adolentesis。 婴儿主要由B.Bifidum和B. Longum殖民。 母亲和她的婴儿在13例中的9例中由相同的双歧杆菌物种殖民。 在这些病例中的5（38.5％）中，这些菌株对它们的REP-PCR谱相同。 这些菌株属于B. Longum在一个病例中，B. Bifidum在3例中，1例中的B. Adolentesis。 我们的结果支持母体双歧杆菌菌株早期殖民化的假设。

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