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首页> 外文期刊>British Journal of Dermatology >Basket‐weave structure in the stratum corneum is an important factor for maintaining the physiological properties of human skin as studied using reconstructed human epidermis and tape stripping of human cheek skin
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Basket‐weave structure in the stratum corneum is an important factor for maintaining the physiological properties of human skin as studied using reconstructed human epidermis and tape stripping of human cheek skin

机译：Stratum Corneum中的篮子编织结构是使用重建的人表皮和人类脸颊皮肤的磁带剥离，在研究人体皮肤的生理特性

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Summary Background The normal stratum corneum ( SC ) has an upper basket‐weave ( BW ) pattern layer and a lower compact layer. The transition from compact to BW SC is well associated with a transition from diffuse to peripheral distributions of corneodesmosomes ( CD s). The loss of transition from compact SC to BW SC appears to cause structural and barrier‐function impairments. Objectives To show the involvement of the BW SC in maintaining the physiological properties of the skin. Methods Reconstructed human epidermis ( RHE ) with a complete BW structure was created by treatment with prepared emulsion‐A, an oil‐in‐water emulsion. The RHE tissues were subjected to histological analysis, and the distribution of CD s on the SC with or without BW SC was analysed by anti‐desmoglein (Dsg)1 antibody immunofluorescence and ultrastructural and Western blotting analyses. Ultrastructural analysis of intercellular lipids was performed. The mechanical properties of the RHE were evaluated. Results Emulsion‐A successfully generated the BW SC in the RHE in which the degradation of CD s was promoted. The intercellular space of the BW SC generated by emulsion‐A was filled with multilamellar lipid sheets. The softness of the SC with a BW structure formed with emulsion‐A was higher than that of the compact SC in RHE . The outermost SC Dsg1 degradation (formation of the BW SC as determined with Dsg1 pixels) was correlated with water‐barrier functions and the SC softness of healthy human cheek, which varied widely. Conclusions Emulsion‐A successfully generated the BW SC in RHE for the first time. This method is suggested to be a useful tool for investigating the physiological significance of the BW SC in?vitro . Determination of Dsg1 content in the SC obtained by tape stripping from human skin allows study of the effects of external stimulants, such as creams and ointments, including cosmetics, on the completeness of the BW SC in?situ without biopsy. What's already known about this topic? The normal stratum corneum (SC) has two layers, an upper basket‐weave (BW) pattern layer and a lower compact layer. Epidermal diseases such as ichthyosis vulgaris and X‐linked ichthyosis have an incomplete or no BW SC and impaired SC barrier functions, in which corneodesmosome (CD) degradation in a peripheral distribution is impaired. The roles of the BW SC in the physiological properties of human skin have not been clearly elucidated. What does this study add? Reconstructed human epidermis (RHE) with a complete BW structure was generated for the first time by treatment with oil‐in‐water emulsion‐A. The formation of the BW SC was associated with a decrease in Dsg1 content, which represents the CD number in the SC. The intercellular space of the BW SC generated by emulsion‐A, but not compact SC, was filled with multilamellar lipid sheets. The softness of the SC with a BW structure formed by emulsion‐A treatment was higher than that of the compact SC in RHE. What is the translational message? RHE with a complete BW SC generated by emulsion‐A treatment is suggested to be a useful tool for investigating effects on the physiological functions of the BW SC, as in treatments with creams and ointments including cosmetics. Determination of desmoglein 1 content in the SC obtained by tape stripping from human skin can make it possible to study the effects of external stimulants, such as creams and ointments, including cosmetics, on the completeness of the BW SC in?situ without biopsy.
机译：发明内容背景正常的角质层（SC）具有上篮式织物（BW）图案层和下紧凑层。从Compact到BW SC的过渡与从漫射到Corneodesmosomes（CDS）的外周分布的转变相关。从Compact SC到BW SC的过渡损失似乎导致结构和障碍功能损伤。目的展示BW SC在保持皮肤的生理特性方面的累积。方法通过用制备的乳液-A，水包油乳液处理来重建具有完整BW结构的人表皮（RHE）。对rhe组织进行组织学分析，通过抗衰减（DSG）1抗体免疫荧光和超微结构和Western印迹分析分析具有或不具有BW SC的SC上CDS的分布。进行细胞间脂质的超微结构分析。评估RHE的机械性能。结果乳液-A成功地产生了RHE中的BW SC，促进了CDS的降解。由乳液-A产生的BW SC的细胞间空间填充有多层脂质片。用乳液-A形成的BW结构的SC的柔软性高于RHE中的紧致SC的BW结构。最外面的SC DSG1劣化（用DSG1像素确定的BW SC的形成）与水屏障功能和健康人脸颊的SC柔软，广泛变化。结论乳液 - A首次在RHE中成功生成了BW SC。该方法建议是一种用于研究体外BW SC的生理意义的有用工具。通过人体皮肤剥离获得的SC中DSG1含量的测定允许研究外部兴奋剂的影响，例如乳膏和软膏，包括化妆品，在没有活组织检查的情况下的BW SC的完整性。这个主题已经知道了什么？正常的角质层（Sc）具有两层，上篮 - 编织（BW）图案层和较低的紧凑层。表皮疾病如查查症和X键性的ICHThthyosis的表皮疾病具有不完全或没有BW SC和受损的SC屏障功能，其中粘土体（CD）在外周分布中的降解受损。 BWS SC在人体皮肤生理特性的作用尚未明确阐明。这项研究添加了什么？通过用水 - 水乳液-A处理首次产生具有完整BW结构的重建人表皮（RHE）。 BWS SC的形成与DSG1含量的减少相关，这表示SC中的CD号。由乳液-A而不是紧凑SC产生的BW SC的细胞间空间填充有多层脂质板。用乳液形成的BW结构的SC的柔软度高于RHE中的紧致SC的BW结构。什么是翻译消息？用乳液产生的完全BW Sc的RHE建议是一种有用的工具，用于研究BW SC的生理功能的影响，如在包括化妆品的乳膏和软膏的治疗中。通过从人体皮肤剥离的胶带剥离获得的SC中的滴定粒1含量可以使得可以研究外部兴奋剂的影响，例如乳膏和软膏，包括化妆品，在没有活组织检查的情况下的BW SC的完整性。

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  • 来源
    《British Journal of Dermatology》 |2020年第2期|共9页
  • 作者

    Goto H.; Tada A.; Ibe A.; Kitajima Y.;

  • 作者单位

    Product Development DepartmentPOLA Chemical Industries Inc.560 Kashio‐cho Totsuka‐ku Yokohama 244;

    Product Development DepartmentPOLA Chemical Industries Inc.560 Kashio‐cho Totsuka‐ku Yokohama 244;

    Frontier Research CenterPOLA Chemical Industries Inc.560 Kashio‐cho Totsuka‐ku Yokohama 244‐0812;

    Department of DermatologyKizawa Memorial Hospital590 Shimokobi Kobi Minokamo Gifu 505‐8503 Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 皮肤病学与性病学;
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