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Triplet-Induced Lesion Formation at CpT and TpC Sites in DNA

机译：在DNA中CPT和TPC位点的三联诱导的病变形成

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摘要

UV irradiation induces DNA lesions particularly at dipyrimidine sites. Using time-resolved UV pump (250 nm) and mid-IR probe spectroscopy the triplet pathway of cyclobutane pyrimidine dimer (CPD) formation within TpC and CpT sequences was studied. The triplet state is initially localized at the thymine base but decays with 30 ns under formation of a biradical state extending over both bases of the dipyrimidine. Subsequently this state either decays back to the electronic ground state on the 100 ns time scale or forms a cyclobutane pyrimidine dimer lesion (CPD). Stationary IR spectroscopy and triplet sensitization via 2'-methoxyacetophenone (2-M) in the UVA range shows that the lesions are formed with an efficiency of approximately 1.5 %. Deamination converts the cytosine moiety of the CPD lesions on the time scale of 10 hours into uracil which gives CPD(UpT) and CPD(TpU) lesions in which the coding potential of the initial cytosine base is vanished.
机译：紫外线辐照诱导DNA病变，特别是在双嘧啶位点。 使用时间分辨的UV泵（250nm）和MID-IR探针光谱，研究了TPC和CPT序列中环丁烷嘧啶二聚体（CPD）形成的三重态途径。 三重态状态最初在胸腺嘧啶基碱中定位，但是在形成在双嘧啶的两个碱基上延伸的荚膜状态下的30ns衰减。 随后，该状态要么在100NS时间标度上衰减回到电子接地状态，或者形成环丁烷嘧啶二聚体病变（CPD）。 通过2'-甲氧基乙酮（2-M）在UVA范围内的固定IR光谱和三重态敏化表明，损伤形成为约1.5％的效率。 脱氨酸将CPD病变的胞嘧啶部分转化为10小时的尿嘧啶的时间等级，所述尿嘧啶给出CPD（UPT）和CPD（TPU）病变，其中初始胞嘧啶碱的编码电位消失。

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  • 来源
    《Chemistry: A European journal》 |2019年第66期|共9页
  • 作者

    Julia Gontcharov; Lizhe Liu; Bert M. Pilles; Thomas Carell; Wolfgang J. Schreier; Wolfgang Zinth;

  • 作者单位

    Lehrstuhl fer BioMolekulare Optik Fakult-t fer Physik and Center for Integrated Protein Science Munich CIPSM Ludwig-Maximilians-Universit-t Menchen Oettingenstr. 67 80538 Menchen (Germany);

    Lehrstuhl fer BioMolekulare Optik Fakult-t fer Physik and Center for Integrated Protein Science Munich CIPSM Ludwig-Maximilians-Universit-t Menchen Oettingenstr. 67 80538 Menchen (Germany);

    Lehrstuhl fer BioMolekulare Optik Fakult-t fer Physik and Center for Integrated Protein Science Munich CIPSM Ludwig-Maximilians-Universit-t Menchen Oettingenstr. 67 80538 Menchen (Germany);

    Center for Integrated Protein Science am Department Chemistry Ludwig-Maximilians-Universit-t Menchen Butenandtstra?e 5-13 81377 Menchen (Germany);

    Lehrstuhl fer BioMolekulare Optik Fakult-t fer Physik and Center for Integrated Protein Science Munich CIPSM Ludwig-Maximilians-Universit-t Menchen Oettingenstr. 67 80538 Menchen (Germany);

    Lehrstuhl fer BioMolekulare Optik Fakult-t fer Physik and Center for Integrated Protein Science Munich CIPSM Ludwig-Maximilians-Universit-t Menchen Oettingenstr. 67 80538 Menchen (Germany);

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 应用化学;
  • 关键词

    cyclobutane pyrimidine dimer; dna damage; photosensitization; time-resolved ir spectroscopy; uv irradiation;

    机译：环丁烷嘧啶二聚体;DNA损伤;光敏化;时间分离的IR光谱;UV辐照;

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