• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chemistry: A European journal >Polyadenine-Modulated DNA Conformation Monitored by Surface-Enhanced Raman Scattering (SERS) on Multibranched Gold Nanoparticles and Its Sensing Application
【24h】

Polyadenine-Modulated DNA Conformation Monitored by Surface-Enhanced Raman Scattering (SERS) on Multibranched Gold Nanoparticles and Its Sensing Application

机译：通过表面增强拉曼散射（SERS）对多刺素金纳米颗粒及其传感施用监测的聚腺嘌呤调节的DNA构象

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This work proposes a facile way to modulate the conformation of DNA from the "Lie-Down" to the "Stand-Up" conformation on the surface of multibranched gold nanoparticles (AuNPs). This is realized by regulating the length of polyadenine (polyA) linked to the DNA sequence and/or the hybridization of this sequence with the target DNA, and can be monitored by the Raman signal owing to the excellent performance of multibranched AuNPs (AuNSs) as a surface-enhanced Raman scattering (SERS) substrate and the distance change between the Raman reporter and the substrate. The probable mechanism, which depends on the repulsion of polyA from the sequence and the tip assembly, has also been probed through theoretical simulation using the finite difference time domain method. By virtue of this strategy, a conformation-transformation-based DNA-AuNS sensor is constructed for the identification of a specific oligonucleotide, which has been used for the detection of DNA sequences associated with Severe Acute Respiratory Syndrome (SARS). This strategy leads to a novel sensing platform with good extendibility for DNA analysis, and provides a powerful protocol for facilitating the cognition of DNA conformation on metal surfaces.
机译：这项工作提出了一种容易方式来调节DNA与“谎言”的构象，以“沉默”在多刺挛性金纳米粒子（AUNP）表面上的“站立”构象上。通过调节与DNA序列的多腺苷（PolyA）的长度和/或通过靶DNA的序列的杂交来实现这一点，可以通过拉曼信号来监测多刺肌（AUNSS）的优异性能。表面增强的拉曼散射（SERS）基板和拉曼报道器和基板之间的距离变化。通过使用有限差分时域方法，还取决于从序列和尖端组件的序列和尖端组件的排斥的可能机构。借助于该策略，构建基于符合转换的DNA-AUNS传感器，用于鉴定特定的寡核苷酸，其已被用于检测与严重急性呼吸综合征（SARS）相关的DNA序列。该策略导致新型的传感平台具有良好的DNA分析可扩展性，并提供了一种强大的协议，以促进DNA构象对金属表面的认知。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号