• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chemistry: A European journal >Luminescent Metal–Organic-Framework-Based Label-Free Assay of Polyphenol Oxidase with Fluorescent Scan
【24h】

Luminescent Metal–Organic-Framework-Based Label-Free Assay of Polyphenol Oxidase with Fluorescent Scan

机译：基于发光的金属 - 有机框架的无荧光氧化酶的无标记测定，具有荧光扫描

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Metal–organic frameworks (MOFs) are emerging in recent years as a kind of versatile fluorescent sensing materials, but their application to enzyme assays has rarely been studied. Here, the first example of a MOF-based label-free enzyme assay system is reported. A luminescent MOF was synthesized and applied to the activity analysis of polyphenol oxidase (PPO). With its distinct responses to the phenolic substrate and o-quinone product, this MOF could transduce the extent of PPO-catalyzed oxidation to fluorescence signal and enable the real-time monitoring of this reaction. Wide substrate adaptability and high sensitivity (detection limit= 0.00012 UmL~(-1)) were exhibited by this method, which meets the requirement of common bioanalysis. Interestingly, by the comparison with molecular capturing reagents, the heterogeneous nature of this MOF-based assay effectively preventing the interaction with the enzyme was proven, thus ensuring the authenticity of results.
机译：近年来，金属有机框架（MOF）是一种多功能荧光传感材料，但它们对酶测定的应用很少。 这里，报道了基于MOF的无标记酶测定系统的第一实例。 合成发光MOF并应用于多酚氧化酶（PPO）的活性分析。 对于对酚醛基质和O-醌产物的不同反应，该MOF可以将PPO催化氧化的程度转化为荧光信号，并能够进行该反应的实时监测。 通过该方法表现出宽底板适应性和高灵敏度（检测限= 0.00012 UML〜（-1）），其符合常见生物分析的要求。 有趣的是，通过与分子捕获试剂的比较，证明了这种基于MOF的测定的非均相性质有效地防止了与酶的相互作用，从而确保了结果的真实性。

著录项

  • 来源
    《Chemistry: A European journal》 |2017年第27期|共8页
  • 作者

    Yue Li; An Guo; Lan Chang; Wen-Juan Li; Wen-Juan Ruan;

  • 作者单位

    College of Chemistry and Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Nankai University Tianjin 300071 (P. R. China);

    College of Chemistry and Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Nankai University Tianjin 300071 (P. R. China);

    College of Chemistry and Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Nankai University Tianjin 300071 (P. R. China);

    College of Chemistry and Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Nankai University Tianjin 300071 (P. R. China);

    College of Chemistry and Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Nankai University Tianjin 300071 (P. R. China);

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 应用化学;
  • 关键词

    enzyme assay; fluorescence; metal-organic frameworks; polyphenol oxidase; sensors;

    机译：酶测定;荧光;金属 - 有机框架;多酚氧化酶;传感器;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号