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首页> 外文期刊>Chemistry: A European journal >Covalently Connected Polymer-Protein Nanostructures Fabricated by a Reactive Self-Assembly Approach
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Covalently Connected Polymer-Protein Nanostructures Fabricated by a Reactive Self-Assembly Approach

机译：通过反应自组装方法制造的共价连接的聚合物 - 蛋白纳米结构

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摘要

The synthesis of polymer-protein nanostructures opens up a new avenue for the development of new biomaterials. In this research, covalently connected polymer-protein nanostructures were fabricated through a reactive selfassembly approach. Poly(tert-butyl methacrylate-co-pyridyl disulfide methacrylamide) (PtBMA-co-PPDSMA) was synthesized by reversible addition fragmentation chain transfer (RAFT) polymerization. Covalently connected nanostructures (CCNs) with hydrophobic polymer cores and hydrophilic protein coronae were prepared by adding solutions of PtBMAco- PPDSMA/DMF to aqueous solutions of bovine serum albumin (BSA). The thiol-disulfide exchange reaction between pyridyl disulfide groups on the polymer chains and thiol groups on the protein molecules plays a key role in the fabrication of CCNs. The self-assembly process was investigated by dynamic light scattering (DLS) and stopped-flow techniques. DLS results indicated that the sizes of the CCNs were determined by the initial polymer concentration, the BSA concentration, and the average number of thiol groups on BSA molecules. TEM and sodium dodecyl sulfate polyacrylamide gel electrophoresis were used to analyze the nanostructures. Far-UV circular dichroism results demonstrated that the original folded conformations of BSA molecules were basically maintained in the reactive self-assembly process. Compared with native BSA, the secondary structure and conformation change of coronal BSA induced by urea or thermal treatment were remarkably suppressed. The cytotoxicity assays demonstrated that the CCNs were essentially nontoxic to Hela and COS-7 cells.
机译：聚合物蛋白纳米结构的合成开辟了新的新生物材料的新途径。在该研究中，通过活性自卸方法制造共价连接的聚合物 - 蛋​​白纳米结构。通过可逆添加碎片链转移（RAFT）聚合，合成聚（甲基丙烯酸叔丁酯 - 二丙吡喃二硫酰甲基丙烯酰胺）（PTBMA-CO-PPDSMA）。通过向牛血清白蛋白（BSA）的水溶液加入Ptbmaco-ppdsma / DMF的溶液来制备具有疏水性聚合物芯和亲水性蛋白质coronae的共价连接的纳米结构（CCN）。在聚合物链中的吡啶基二硫化物基团与蛋白质分子上的硫醇基团之间的二硫化物交换反应在CCN的制造中起着关键作用。通过动态光散射（DLS）和停止流动技术来研究自组装过程。 DLS结果表明，CCN的尺寸由初始聚合物浓度，BSA浓度和BSA分子上的硫醇基的平均数量确定。使用TEM和十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析纳米结构。 FAR-UV圆形二色性结果表明，BSA分子的原始折叠构象基本保持在反应自组装过程中。与天然BSA相比，尿素或热处理诱导的冠状BSA的二级结构和构象变化显着抑制。细胞毒性测定证明CCN基本上与Hela和COS-7细胞基本上无毒性。

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  • 来源
    《Chemistry: A European journal》 |2017年第14期|共9页
  • 作者

    Yuanyuan Ju; Cheng Xing; Dongxia Wu; Yunfang Wu; Lianyong Wang; Hanying Zhao;

  • 作者单位

    Key Laboratory of Functional Polymer Materials Ministry of Education College of Chemistry Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University (P.R. China);

    Key Laboratory of Bioactive Materials Ministry of Education College of Life Sciences Nankai University (P.R. China);

    The Institute of Seawater Desalination and Multipurpose Utilization SOA Tianjin 300192 (P.R. China);

    The Institute of Seawater Desalination and Multipurpose Utilization SOA Tianjin 300192 (P.R. China);

    Key Laboratory of Bioactive Materials Ministry of Education College of Life Sciences Nankai University (P.R. China);

    Key Laboratory of Functional Polymer Materials Ministry of Education College of Chemistry Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University (P.R. China);

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 应用化学;
  • 关键词

    nanostructures; polymers; proteins; selfassembly; synthesis design;

    机译：纳米结构;聚合物;蛋白质;自主;合成设计;

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