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首页> 外文期刊>Chemistry: A European journal >Excess Electron Transfer through DNA Duplexes Comprising a Metal-Mediated Base Pair
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Excess Electron Transfer through DNA Duplexes Comprising a Metal-Mediated Base Pair

机译：通过包含金属介导的碱基对的DNA双链体过量的电子转移

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摘要

Excess electron transfer through one set of DNA duplexes comprising either one or two metal-mediated thymine-HgII-thymine base pairs was studied. Towards this end, the metal-mediated base pair(s) were introduced between an artificial nucleoside bearing a N,N,N',N'-tetramethyl-1,5-diaminonaphthalene derivative (dN, acting as a photoinducible electron donor) and 5-bromo-2'-deoxyuridine (dB, acting as an electron acceptor). Upon oneelectron reduction, dB loses a bromide ion. The amount of unreacted dB remaining after irradiation-determined by LC/ICP-MS-was used to evaluate the charge-transfer efficiency across the metal-mediated base pair(s). Reference measurements with canonical adenine:thymine base pairs prove the applicability of this approach for the detection of charge transfer in DNA. The data indicate that, for the set of DNA duplexes under investigation, excess electron transfer across a thymine-HgII-thymine base pair proceeds with low efficiency, comparable to the transfer across a thymine:thymine mispair. Two contiguous thymine-HgII-thymine base pairs effectively shut down excess electron transfer.
机译：通过一组通过一组DNA双链体通过包含一种或两种金属介导的胸腺嘧啶-HGII-胸腺嘧啶碱基对的过量电子传递。朝向该目的，将金属介导的碱基对引入轴承N，N，N'，N'-四甲基-1,5-二氨基萘衍生物（DN，用作光明性电子供体的DN）和5-溴-2'-脱氧尿苷（DB，用作电子受体）。在一次再调电时，DB失去溴离子。通过LC / ICP-MS照射后剩余的未反应DB的量用于评估金属介导的碱基对的电荷转移效率。用规范腺嘌呤参考测量：胸腺嘧啶基对证明这种方法在DNA中检测电荷转移的适用性。数据表明，对于在调查的DNA双工组中，通过胸腺嘧啶-HGII-胸腺嘧啶基对的过量电子转移以低效率进行，与胸腺嘧啶的转移相当：胸腺嘧啶错误。两种连续的胸腺嘧啶-HGII-胸腺嘧啶基对有效地关闭过量的电子转移。

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  • 来源
    《Chemistry: A European journal》 |2017年第43期|共5页
  • 作者

    Susanne Hensel; Kevin Eckey; Philipp Scharf; Nicole Megger; Uwe Karst; Jens Meller;

  • 作者单位

    Westf-lische Wilhelms-Universit-t Menster Institut fer Anorganische und Analytische Chemie Corrensstra?e 30 48149 Menster (Germany);

    Westf-lische Wilhelms-Universit-t Menster Institut fer Anorganische und Analytische Chemie Corrensstra?e 30 48149 Menster (Germany);

    Westf-lische Wilhelms-Universit-t Menster Institut fer Anorganische und Analytische Chemie Corrensstra?e 30 48149 Menster (Germany);

    Westf-lische Wilhelms-Universit-t Menster Institut fer Anorganische und Analytische Chemie Corrensstra?e 30 48149 Menster (Germany);

    Westf-lische Wilhelms-Universit-t Menster Institut fer Anorganische und Analytische Chemie Corrensstra?e 30 48149 Menster (Germany);

    Westf-lische Wilhelms-Universit-t Menster Institut fer Anorganische und Analytische Chemie Corrensstra?e 30 48149 Menster (Germany);

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 应用化学;
  • 关键词

    charge transfer; DNA; mercury; metal-mediated base pair; thymine;

    机译：电荷转移;DNA;汞;金属介导的碱基对;胸腺嘧啶;

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