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首页> 外文期刊>Chemistry: A European journal >The Effect of Adenine Repeats on G-quadruplex/hemin Peroxidase Mimicking DNAzyme Activity
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The Effect of Adenine Repeats on G-quadruplex/hemin Peroxidase Mimicking DNAzyme Activity

机译：腺嘌呤重复对拟锰活性的G-Quadreplex /血葱过氧化物酶的影响

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摘要

The catalytic activity of G-quadruplex/hemin is much lower than that of proteinous enzymes, so it is very important to increase its activity. Very recently, flanking sequences, which can be regarded as an external part of Gquadruplexes, were found to enhance the activity of Gquadruplex/ hemin DNAzyme. However, little is known about the effect of internal parts, such as loop sequences and linkers, on the activity. In the present study, adenine repeats were incorporated into several designed G-quadruplex structures either in the loops, bulges, or linkers, and the constructed G-quadruplex/hemin DNAzyme exhibit about fivefold improvement in peroxidase-mimicking activity in some cases. The enhancement effect may result from the formation of compound I, protoporphyrin·Fe~(IV)=O~(·+), accelerated by dA repeats, which was demonstrated by H_2O_2 decay kinetics and pH dependency analysis. The novel enhancement methods described here may help in the development of high-activity DNAzymes, illustrated by a dimer G-quadruplex with flanking adenine at one end, a relatively long adenine run in one loop, and another adenine run in the linker.
机译：G-Quadreplex / hemin的催化活性远低于蛋白酶酶的催化活性，因此增加其活性非常重要。最近，发现可以被视为GquadrupleSes的外部的侧翼序列来增强Gquadreplex / Hemin dnazyme的活性。然而，关于活动的内部部件（例如环序列和接头）对活动的影响很少。在本研究中，将腺嘌呤重复掺入环，凸起或接头中的几种设计的G-Quadwlex结构中，并且在某些情况下，构建的G-Quadreplex / Hemin dnazyme表现出过氧化物酶 - 模拟活性的五倍改善。增强效果可能是由化合物I的形成，原子卟啉·Fe〜（IV）= O〜（·+），由DA重复加速，由H_2O_2衰减动力学和pH依赖性分析证明。这里描述的新型增强方法可以有助于开发高活性DNAzymes，通过二聚体G-四重复与一端的侧翼腺嘌呤所示，在一个环中的相对长的腺嘌呤运行，并且在接头中的另一个腺嘌呤运行。

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  • 来源
    《Chemistry: A European journal》 |2017年第17期|共6页
  • 作者

    Jielin Chen; Yuehua Guo; Jun Zhou; Huangxian Ju;

  • 作者单位

    State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing 210023 (China);

    State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing 210023 (China);

    State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing 210023 (China);

    State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing 210023 (China);

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 应用化学;
  • 关键词

    DNA structures; DNAzyme; peroxides; pi interactions; porphyrinoids;

    机译：DNA结构;DNAzyme;过氧化物;PI相互作用;卟啉曲线;

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