...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Current Biology: CB >A Conserved Role of the Unconventional Myosin 1d in Laterality Determination
【24h】

A Conserved Role of the Unconventional Myosin 1d in Laterality Determination

机译：非传统肌球蛋白1D在横向决定中的保守作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Anatomical and functional asymmetries are widespread in the animal kingdom [1,2]. In vertebrates, many visceral organs are asymmetrically placed [3]. In snails,shells and inner organs coil asymmetrically, and in Drosophila,genitalia and hindgut undergo a chiral rotation during development. The evolutionary origin of these asymmetries remains an open question [1]. Nodal signaling is widely used [4],and many,but not all,vertebrates use cilia for symmetry breaking [5]. In Drosophila, which lacks both cilia and Nodal,the unconventional myosin ID (myo1d) gene controls dextral rotation of chiral organs [6,7]. Here,we studied the role of myo1d in left-right (LR) axis formation in Xenopus. Morpholino oligomer-mediated myo1d downregulation affected organ placement in &50% of morphant tadpoles. Induction of the left-asymmetric Nodal cascade was aberrant in &70% of cases. Expression of the flow-target gene dand5 was compromised,as was flow itself,due to shorter,fewer,and non-polarized cilia at the LR organizer. Additional phenotypes pinpointed Wnt/planar cell polarity signaling and suggested that myo1d,like in Drosophila [8],acted in the context of the planar cell polarity pathway. Indeed,convergent extension of gastrula explant cultures was inhibited in myo1d morphants,and the ATF2 reporter gene for non-canonical Wnt signaling was downregulated. Finally,genetic interference experiments demonstrated a functional interaction between the core planar cell polarity signaling gene vangl2 and myo1d in LR axis formation. Thus,our data identified myo1d as a common denominator of arthropod and chordate asymmetry, in agreement with a monophyletic origin of animal asymmetry.
机译：Anathomical和功能性的不对称是动物王国的普遍[1,2]。在脊椎动物中，许多内脏器官不对称地放置[3]。在蜗牛，壳体和内脏器官卷线上不对称，在果蝇，生殖器和后果在开发期间经历手性旋转。这些不对称的进化起源仍然是一个公开的问题[1]。节点信号传导广泛使用[4]，许多，但不是全部，脊椎动物使用纤毛用于对称性断裂[5]。在果蝇缺乏纤毛和节点的果蝇中，非传统的肌球蛋白ID（MyO1D）基因控制手性器官的右侧旋转[6,7]。在这里，我们研究了Xenopus左右左右（LR）轴形成的MyO1d的作用。吗啉氨基寡聚蛋白介导的Myo1d下调受影响器官放置在＆amp; 50％的Merphant Tadpoles。左不对称节点级联的诱导是在＆amp中的异常中的。 70％的病例。由于LR组织者的较短，更少和非极化的纤毛，流动本身，流动靶基因丹丹5的表达受到损害。额外的表型针对WNT /平面细胞极性信号传导，并表明MyO1d在果蝇[8]中，在平面细胞极性途径的上下文中作用。实际上，在MyO1D鱼中抑制了胃肠外植物培养物的收敛延伸，并且对非规范性WNT信号传导的ATF2报告基因进行了下调。最后，遗传干扰实验证明了LR轴形成中的核心平面细胞极性信号传导基因Vangl2和MyO1d之间的功能相互作用。因此，我们的数据将Myo1d确定为节肢动物和脊索不对称的常见分母，与动物不对称的单一的血统起源一致。

著录项

  • 来源
    《Current Biology: CB》 |2018年第5期|共10页
  • 作者

    Tingler Melanie; Kurz Sabrina; Maerker Markus; Ott Tim; Fuhl Franziska; Schweickert Axel; LeBlanc-Straceski Janine M.; Noselli Stephane; Blum Martin;

  • 作者单位

    Univ Hohenheim Inst Zool Garbenstr 30 D-70593 Stuttgart Germany;

    Univ Hohenheim Inst Zool Garbenstr 30 D-70593 Stuttgart Germany;

    Univ Hohenheim Inst Zool Garbenstr 30 D-70593 Stuttgart Germany;

    Univ Hohenheim Inst Zool Garbenstr 30 D-70593 Stuttgart Germany;

    Univ Hohenheim Inst Zool Garbenstr 30 D-70593 Stuttgart Germany;

    Univ Hohenheim Inst Zool Garbenstr 30 D-70593 Stuttgart Germany;

    Merrimack Coll Dept Biol 315 Turnpike St N Andover MA 01845 USA;

    Univ Cote dAzur CNRS INSERM Inst Biol Valrose Parc Valrose F-06108 Nice France;

    Univ Hohenheim Inst Zool Garbenstr 30 D-70593 Stuttgart Germany;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物科学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号