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Expression of early nodulin promoter gene in transgenic rice

机译:早期Nodulin启动子基因在转基因水稻中的表达

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We obtained transgenic rice plants carrying the alfalfa (Medicago sativa) Msenod 12 promoters fused to the uidA (GUS) reporter gene. While transient expression of the constructs was not detectable, the Msenod 12B promoter was expressed in the stable rice transformants. GUS activity was detected in proembryogenic calli, whereas in the regenerated plants, without external supply of enod 12 inducers, it was found in the root vascular tissue. This expression pattern was changed, however, in the transgenic rice roots maintained in a 2,4-D-containing medium by microballistic application of the Nod factor, NodRm-IV (C16 : 2,S) from Rhizobium meliloti, the microsymbiont of M. sativa.
机译:我们获得携带苜蓿(Medicago Sativa)MSENOD 12启动子与UIDA(GUS)报告基因融合的转基因水稻植物。 虽然构建体的瞬时表达是不可检测的,但MSENOD 12B启动子在稳定的水稻转化体中表达。 在Proembergencogical Calli中检测到GUS活性,而在再生植物中,没有Enod 12诱导剂的外部供应,在根血管组织中发现。 然而,这种表达模式改变在转基因水稻根中,通过微型施用Nod系数,Nodrm-IV(C16:2,S)从rhizobium Meliloti,MicroMbiont的Microballative施用 。苜蓿。

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