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Morphological, cytological, palynological and molecular characterization of certain Mangifera species

机译:某些人格因素物种的形态学,细胞学,椎相论和分子表征

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The Mangifera genus has more than 60 species, mostly distributed in tropical Asia. The wild relatives of Mangifera are considered reservoirs of potential genes that can confer tolerance/resistance to biotic and abiotic stresses. The morphological, cytological and molecular characterization of eight species was done to study the diversity and phylogenetic relationship among different Mangifera species. In order to study the evolutionary relationship and polymorphism among the mango species, the ITS1/ITS4 gene and partial chloroplast psbH-trnH genes were sequenced. Phylogenetic analysis of the nuclear and chloroplast marker revealed that the M. indica L. is closely related to M. griffithii and M. camptosperma, which belong to subgenus Mangifera. Results indicate that the taxonomic position of M. andamanica should be reconsidered as this species is very close to Bouea oppositifolia which is evident from both ITS and psbA-trnH rDNA analysis. The morphological traits such as tree, leaf, flowers and fruits and palynological and cytology of the genus mango were used to distinguish the species and its phylogentic status. The morphological traits among various species indicate the high level of variability which were further confirmed with ITS sequences and cpDNA. Phylogenetic analysis illustrates that partial chloroplast psbH-trnH gene gave better polymorphism in mango species than nuclear ITS. The pollen morphology and chromosomal counts were also done in certain Mangifera species to study the phylogenetic relationship.
机译:Mangifera属的物种具有超过60种,主要分布在热带亚洲。 Mangifera的野生亲属被认为是可以赋予​​生物和非生物胁迫的耐受性/抗性的潜在基因的储层。完成了八种物种的形态学,细胞学和分子表征,以研究不同人格因素之间的多样性和系统发育关系。为了研究芒果物种之间的进化关系和多态性,测序其1 / ITS4基因和部分叶绿体PSBH-TRNH基因。核和叶绿体标记物的系统发育分析显示,M. Indica L.与M. Griffithii和M. camppoSperma密切相关,属于脓性人类。结果表明,应重新考虑M.Andamanica的分类学位置,因为该物种非常接近Bouea对比,这是从其和PSBA-TRNH RDNA分析中明显的。诸如树,叶,花和水果等形态特征,用于区分物种及其文学状态。各种物种中的形态学性状表明,用其序列和CPDNA进一步证实了高含量的可变性。系统发育分析说明部分叶绿体PSBH-TRNH基因在芒果物种中具有比核的更好的多态性。花粉形态和染色体计数也在某些人口膜物种中进行,以研究系统发育关系。

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