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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Calcite precipitation by Rhodococcus sp isolated from Kotumsar cave, Chhattisgarh, India
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Calcite precipitation by Rhodococcus sp isolated from Kotumsar cave, Chhattisgarh, India

机译:由rhodococcus sp的方解石沉淀从Kotumsar洞,Chhattisgarh,印度

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摘要

The precipitation of carbonate minerals by Rhodococcus sp. strain S14 isolated from Kotumsar cave, Chhattisgarh, India is reported. The speleothems at Kotumsar showed high microbial cell enumeration on B4 agar; iron agar (3.4 x 10(5) CFU/g) and sulphite agar (7.2 x 10(2) CFU/g). National Centre for Biotechnology Information database was used for the BLASTn sequence search of 16S rRNA sequences. The S14 strain gave similarity scores of &= 99% with the respective organisms on the database. The strain was identified as Rhodococcus sp. Culture experiments performed using the isolated strains suggested that the rate of precipitation was dependent on pH, temperature and bacterial growth. Rhodococcus sp. S14 strain induced the formation of calcite in vitro and the biominerals produced were calcified spherulites with pores ( as imaged with SEM). The precipitate, at the end of the experimental period of 35 days, had the appearance of coccoliths. This is the initial report on the possible involvement of Rhodococcus sp. in the precipitation of carbonates at Kotumsar cave.
机译:rhodococcus sp碳酸盐矿物的沉淀。据报道,从Kotumsar Cave,Chhattisgarh菌株S14被报道。 Kotumsar的Speleothss在B4琼脂上显示出高微生物细胞枚举;铁琼脂(3.4×10(5)CFU / g)和亚硫酸盐琼脂(7.2×10(2)CFU / g)。国家生物技术信息数据库用于BLASTN序列搜索16S rRNA序列。 S14菌株给出了相似性的分数& = 99%,与数据库上的各自有机体。将该菌株鉴定为rhodococcus sp。使用分离的菌株进行的培养实验表明,沉淀速率取决于pH,温度和细菌生长。 rhodococcus sp。 S14菌株在体外形成方解石的形成,并且产生的生物体煅烧孔隙晶胞孔(用SEM成像)。在35天的实验期结束时,沉淀物具有Coccoliths的出现。这是关于rhodococcus sp可能参与的初步报告。在Kotumsar洞穴的碳酸盐沉淀中。

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