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首页> 外文期刊>Acta Horticulturae >Partial characterization of two new viruses in ash belonging to the families Partitiviridaeand Caulimo viridae
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Partial characterization of two new viruses in ash belonging to the families Partitiviridaeand Caulimo viridae

机译：两种新病毒属于家庭的两种新病毒的局部表征Partitiviridaeand Caulimo Viridae

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摘要

Ash trees (Fraxinus spp.) are widely distributed through European, Asian, and North American temperate zones. There are numerous recently identified pests and pathogens that have killed hundreds of millions of ash worldwide. In 2014, white ash trees in Colorado exhibited symptoms of chlorotic patches that increased in size through the growing season and developed necrotic lesions. Partially purified extracts of symptomatic leaves examined by transmission electron microscopy identified spherical virus-like particles. Next generation RNA sequencing of symptomatic tissue identified five virus-like contigs with similarity to both partitiviruses and caulimoviruses. Primers designed from the putative contigs amplified products of the expected size from both inoculated plants and wild ash seedlings. Further work is required to complete the virus genomes, phylogenetic analysis, complete Koch's postulates, and determine the distribution of these viruses in the Fraxinus genus.
机译：灰树（Fraxinus SPP）广泛分布通过欧洲，亚洲和北美温带区。 最近鉴定的害虫和病原体在全球造成数亿次灰烬。 2014年，科罗拉多州的白灰树展出了氯化斑块的症状，通过生长季节增加了大小，并发育坏死病变。 通过透射电子显微镜检查的症状叶片的部分纯化提取物鉴定了球形病毒样颗粒。 症状组织的下一代RNA测序鉴定了与Partiviruses和CauliMovirus相似的五种病毒样谱。 由推定的Contigs设计的引物从接种植物和野生灰幼苗中扩增的预测尺寸的扩增产物。 需要进一步的工作来完成病毒基因组，系统发育分析，完整的KOCH的假设，并确定在Fraxinus Genus中这些病毒的分布。

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