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首页> 外文期刊>Acta Horticulturae >The use of chloroplast gene sequences to confirm maternal backgrounds of powdery mildew resistant, putatively interspecific hybrid dogwood [Cornuskousa x c. florida) seedlings
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The use of chloroplast gene sequences to confirm maternal backgrounds of powdery mildew resistant, putatively interspecific hybrid dogwood [Cornuskousa x c. florida) seedlings

机译：使用叶绿体基因序列来确认抗性抗性抗性抗性，借助于特异性杂交山茱萸的母体背景[Cornuskousa x C. 佛罗里达州）幼苗

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摘要

Powdery mildew (PM), caused by both Erysiphe pulchra Cook and Peck and Phyllactinia guttata (Wall.:Fr) Lev., is a major fungal disease of flowering dogwood (Cornus florida L.) and genetic resistance to the pathogen is rare in the species. At Rutgers we have identified a population of 11 resistant seedling trees which phenotypically appear to be pure C. florida, but their pedigree records indicate that they are advanced generation backcross hybrids with C. kousa F. Buerger ex Hence. Several chloroplast genes including matK, rbcL and ycfl have been used in phylogenetic analysis of the Cornaceae family. They have also been used to determine maternal species backgrounds of interspecific hybrid progeny of higher plants. In this study we utilize chloroplast genes to examine the maternal species of our powdery mildew resistant seedlings with the expectation of confirming C. kousa as the maternal source. Chloroplast genes were sequenced in the 11 seedlings along with three reference cultivars of C. kousa and four of C. florida. The recorded mother of the PM-resistant seedlings, breeding selection KF95-1 (a BCi hybrid between C. kousa x C. florida crossed back to C. florida), was also included in the study. Further, two offspring from one of the PM-resistant seedlings crossed again to C. florida were included to trace the maternal line into the BC3 generation. We were able to successfully sequence the chloroplast genes for all genotypes included in the study, but had confounding sequencing results for theC. kousa controls. Nonetheless, results showed that while our pedigree records indicate that our PM-resistant seedlings trace their maternal lines to C. kousa 'K2', this is most likely in error. Gene sequences indicate that the seedlings were derived from C. florida maternal lines. While the results are humbling, they reinforce the value of supporting breeding records with molecular tools. Regardless of the origin of disease resistance, the presence of heritable PM resistance in C. florida holds significant potential for breeding.
机译：粉末状霉菌（PM），由erysiphe pulchra cook和peck和phyllactinia guttata（墙壁.: forf）Lev。，是开花山茱萸（Cornus florida L.）的主要真菌疾病，以及病原体的遗传性是罕见的物种。在Rutgers，我们已经确定了11种抗性幼苗树木的人口，这表型似乎是纯C.佛罗里达州，但它们的血统记录表明它们是先进的回复杂种，与C.KOUSA F. Buerger Ex。包括MATK，RBCL和YCFL在内的几种叶绿体基因已被用于山茱萸的系统发育分析。他们还被用来确定高等植物的杂交后代的母体物种背景。在这项研究中，我们利用叶绿体基因来检查我们的粉末状霉菌耐药幼苗的母体物种，期望确认C.KOUSA作为母体源。在11个幼苗中，在11种幼苗中测序叶绿体基因，以及C.KOUSA的三种参考品种和C.佛罗里达州的四种参考品种。目前的幼苗的母亲，繁殖选择KF95-1（C.Kousa X C. Florida之间的BCI杂交越过弗洛里达，佛罗里达州的BCI杂交）也包括在研究中。此外，将来自其中一个耐幼苗的两个后代再次交叉至佛罗里达州C.佛罗里达州以将母线呈现为BC3的生成。我们能够成功地序列研究中包含的所有基因型的叶绿体基因，但对THEC具有混淆的测序结果。 KOUSA控制。尽管如此，结果表明，虽然我们的血统记录表明我们的PM耐药幼苗追踪其母线至C.KOUSA'K2'，这是最有可能的。基因序列表明幼苗衍生自C.佛罗里达母线。虽然结果是羞辱，但它们加强了用分子工具支持育种记录的价值。无论抗病的起源如何，C.佛罗里达州C.抗性PM抗性的存在具有显着的繁殖潜力。

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  • 来源
    《Acta Horticulturae》 |2018年第1191期|共10页
  • 作者

    M. Muehlbauer; J. Honig; T. Molnar;

  • 作者单位

    Rutgers The State University of New Jersey Department of Plant Biology and Pathology 59 Dudley Road New Brunswick NJ USA;

    Rutgers The State University of New Jersey Department of Plant Biology and Pathology 59 Dudley Road New Brunswick NJ USA;

    Rutgers The State University of New Jersey Department of Plant Biology and Pathology 59 Dudley Road New Brunswick NJ USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 园艺;
  • 关键词

    matK; rbcL; ycfl; Erysiphe pulchra; Phyllactinia guttata;

    机译：MATK;RBCL;YCFL;erysiphe美丽;Phyllactinia gnttata;

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