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首页> 外文期刊>Acta Horticulturae >Microsatellites as a tool for identifying successful pollinators of the pear cultivar 'Ingeborg' in Ullensvang, Norway
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Microsatellites as a tool for identifying successful pollinators of the pear cultivar 'Ingeborg' in Ullensvang, Norway

机译：MicroSatellites作为挪威Ullensvang中梨品种“ingeborg”成功粉碎者的工具

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摘要

Due to their Mendelian inheritance, microsatellites or SSRs (simple sequence repeats) can readily be used for parentage analyses of pear seedlings, thus revealing the female and male parents of the plant. However, in cases where obtained pear seeds display low viability, conducting a parentage analyses on the pear seeds themselves could be used in order to identify the male parent (successful pollinator). In this study, batches of seeds were extracted from fruits of the triploid 'Ingeborg' pear ('Conference' x 'Bonne Louise'), the main commercial pear cultivar in Norway. Mature pears were collected from eight commercial pear orchards located in Ullensvang, at 60° North and seeds were collected. Genomic DNA was subsequently isolated from the obtained seed batches and genotyped using 12 microsatellite markers. The same markers were also used for the genetic characterization of 'Ingeborg' and five pear genotypes used as pollinators in the sampled orchards ('Clara Frijs', 'Herzogine Elsa', 'Anna', 'Coloree de Juillet' and 'Belle lucrative'). The obtained SSR profiles were used in paternity analyses, as well as for gene assignment analyses. Both approaches identified pear 'Clara Frijs' to be the most successful pollinator in most of the sampled orchards. Subsequent S allele genotyping of 'Ingeborg' and five pear genotypes used as pollinators in the sampled orchards revealed that the preferential pollination by individual genotypes was not caused by gametophytic incompatibility or semi-compatibility.
机译：由于它们的孟德尔遗传，微卫星或SSRS（简单序列重复）可以容易地用于梨幼苗的父母分析，从而揭示植物的女性和雄性父母。然而，在所获得的梨子种子显示低可行性的情况下，可以使用在梨子种子上进行父母分析，以便识别雄性父母（成功的Collinator）。在这项研究中，从挪威的主要商业梨品种，从三倍体'ingborg'梨的果实中提取种子批量种子。成熟的梨从位于Ullensvang的八个商用梨果园收集，北北北部和种子被收集。随后从所得的种子批留分离和使用12微卫星标记的基因分型分离基因组DNA。相同的标记也用于“IngeBorg”和5梨基因型的遗传表征，用作样品园（'Clara Frijs'，'Herzogine Elsa'，'Anna'，'Coloree de Juillet'和'Belle Lucrived'） ）。所获得的SSR型材用于亲子鉴定，以及基因分配分析。两种方法都认为梨'Clara Frijs'是大多数采样的果园中最成功的粉碎机。随后的S等位基因基因分型在采样的果园中用作粉丝簇的五梨基因型，揭示了单个基因型的优先授粉不是由配子蛋白不相容性或半相容性引起的。

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  • 来源
    《Acta Horticulturae》 |2018年第1229期|共4页
  • 作者

    M. Meland; M. Kurtovic; B. Kalamujic; N. Pojskic; L. Lasic; F. Gasi;

  • 作者单位

    Norwegian Institute of Bioeconomy Research (NIBIO) Ullensvang Norway;

    Faculty of Agriculture and Food Sciences University of Sarajevo Zmaja od Bosne 8 71 000 Sarajevo Bosnia and Herzegovina;

    Laboratory for Molecular Genetics of Natural Resources Institute for Genetic Engineering and Biotechnology Zmaja od Bosne 8 Kampus 71 000 Sarajevo Bosnia and Herzegovina;

    Laboratory for Molecular Genetics of Natural Resources Institute for Genetic Engineering and Biotechnology Zmaja od Bosne 8 Kampus 71 000 Sarajevo Bosnia and Herzegovina;

    Laboratory for Molecular Genetics of Natural Resources Institute for Genetic Engineering and Biotechnology Zmaja od Bosne 8 Kampus 71 000 Sarajevo Bosnia and Herzegovina;

    Faculty of Agriculture and Food Sciences University of Sarajevo Zmaja od Bosne 8 71 000 Sarajevo Bosnia and Herzegovina;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 园艺;
  • 关键词

    Pyrus communis L.; pear seeds; gene assignment analyses; gametophytic incompatibility;

    机译：Pyrus Communis L.;梨子种子;基因分配分析;杂草类药不相容;

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