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首页> 外文期刊>Acta Horticulturae >Determination of the effects of salt stress in spinach by MRI and CT imaging techniques
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Determination of the effects of salt stress in spinach by MRI and CT imaging techniques

机译：用MRI和CT成像技术测定菠菜中盐胁迫的影响

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The research was carried out to determine the changes occurred on the spinach leaf tissues as a result of salt stress by using MRI (Magnetic Resonance Imaging) and CT (Computed Tomography). In study which were used the 'Meridien Fi' and 'San Moreno Fi' as plant material, plants were grown in hydroponic system including Hoagland nutrient solutions under controlled conditions. For this purpose, the cultivars were grown in a climate chamber at 22/18°C (day/night) temperature, 70% relative humidity, 10/14 h (light/night) photoperiod and under 400 umol nr2 s1 light intensity conditions. Salt applications have been started at the period which plants had 4-5 true leaves and continued until harvest. The salinity of the nutrient solution in the pot was setup with NaCl. Trial were established as randomized plots design with 3 replications and at each replication consisted of two varieties and ('Meridien fi and 'San Moreno Fi') cultivars and 4 different salt concentrations [(EC = control (2), 6, 8 and 10 dSnr1)] were applied. Imaging studies were performed with MRI (magnetic resonance imaging) and CT (computed tomography). MRI images were obtained by taking coronal and axial reference planes of the leaves using Tl weighted (T1W) С + and proton weighted (PDW) sequences in GE Healthcare Brand (General Electric, UK) 1.5 Tesla MR device. In CT imaging, General Electric (UK) the Bright Speed Model with 16 detectors device was used. Sections taken at 0.625 mm diameter, 80 kV and 10 mA values following the angio protocol were examined and then with the help of postprocess multiplanar and volume rendering observations, the images were obtained. The most developed leaves of 3 plants at harvesting period were chosen and examined for the study. According to the observations of MRI and CT images, it was determined that the leaf texture of 'Meridian Fi' against salt stress was less deformed than the leaf texture of 'San Moreno Fi' and at the same time, the water transmission of 'Meridian Fi' was better than 'San Moreno'. By this research, it was shown that the damage caused due to salt stress could be determined in the early period by using MRI and CT imaging.
机译：通过使用MRI（磁共振成像）和CT（计算机断层扫描），进行该研究以确定由于盐应激而在菠菜叶组织上发生的变化。在使用“Meridien Fi”和“San Moreno Fi”的研究中，植物在受控条件下包括Hoagland营养溶液的水培系统中生长。为此目的，在22/18℃（日/夜）温度下，70％相对湿度，10/14小时（光/夜）光周期和400μlNR2 S1光强度条件下，该品种在气候室中生长。盐应用已经开始于植物4-5个真实叶子的时间，并继续直到收获。用NaCl设置罐中营养溶液的盐度。试验被建立为随机地块设计，其中3种复制和每次复制，由两个品种和（'Meridien Fi和'San Moreno Fi'）品种和4种不同的盐浓度（EC =控制（2），6,8和10）组成应用了DSNR1）。用MRI（磁共振成像）和CT（计算机断层扫描）进行成像研究。通过使用GE Healthcare品牌（General Electric，UK）1.5 Tesla MR器件的TL加权（T1W）С+和质子加权（PDW）序列来获得叶片的冠状和轴向参考平面来获得MRI图像。在CT成像中，使用一般电气（英国）具有16个探测器装置的明亮速度模型。检查血管协议后的0.625毫米直径为0.625毫米和10mA值，然后在后处理多平坦和体积渲染观察的帮助下，获得图像。选择和检查在收获时期的3种植物最开发的叶片进行研究。根据MRI和CT图像的观察，确定对盐胁迫的“经络Fi”的叶片纹理比“圣莫雷诺Fi”的叶子纹理变形，同时，“经络的水传输” fi'比'San Moreno'更好。通过本研究，表明通过使用MRI和CT成像，可以在早期确定由于盐应激引起的损伤。

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