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首页> 外文期刊>Acta Horticulturae >The transgenic Dendrob/um'Son.' BOM 17 containing an antisense ACOgene demonstrated normal growth with prolonged vase life
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The transgenic Dendrob/um'Son.' BOM 17 containing an antisense ACOgene demonstrated normal growth with prolonged vase life

机译:转基因Dendrob / Um'son a。' 含有反义Asogene的BOM 17表现出正常的植物生命

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摘要

The growth of four transgenic Dendrobium Sonia 'BOM 17' lines possessing antisense ACC oxidase gene, were evaluated using two-year-old transgenic orchid lines grown in a biosafety greenhouse. The parameters investigated, in relation to ACO included, 1-aminocyclopropane-l-carboxylic acid (ACC) enzyme activity, ACC oxidase (ACO) and ethylene production in the leaves and inflorescence, and the vase life of the inflorescence. The results revealed that the leaves of the transgenic orchid contained 0.15 pmol g-1 FW of ACC while the 4 transgenic lines had ACC levels ranging from 0.1 to 0.15 pmol g1 FW. The ACO enzyme activity in the leaves of the transgenic orchids was found to be in the range of 0.004 to 0.0065 pL C2H4 mg1 protein h1, which was lower thanthose of the non-transgenic lines (0.008), a reduction of 18.75-50%. The transgenic lines produced 0.036 to 0.08 nL C2H4 g1 h1 of ethylene, which was lower than that of the control line at 0.104 nL C2H4 g1 h1, a reduction of 23.08-68.36%. Similar patterns were also observed in the inflorescence. When compared to the non-transgenic line, the ACO activity was reduced to 34.48-53.45% of the control and the ethylene production was not only decreased in the transgenic lines but its production was delayed. The transgenic lines had a 29-57% longer vase life than did the non-transgenic lines.
机译:使用在生物安全温室生长的两岁的转基因兰花线评估具有反义Acc氧化酶基因的四种转基因石斛索尼娅的生长。研究的参数与ACO相关,1-氨基环丙烷-1-羧酸(ACC)酶活性,ACC氧化酶(ACO)和叶片和花序中的乙烯产生,以及花序的花瓶寿命。结果表明,转基因兰花的叶片含有0.15pmol g-1 fw的acc,而4转基因系具有0.1至0.15pmol g1 fw的Acc水平。发现转基因兰花的叶片中的ACO酶活性在0.004至0.0065p1的C 2 H 4 Mg1蛋白H1的范围内,其较低的非转基因系(0.008)的黄色糖,降低18.75-50%。转基因系产生0.036至0.08nl C 2 H 4 G1 H1的乙烯,低于对照管线的0.104nl C 2 H 4 G1 H1,减少23.08-68.36%。在花序中也观察到类似的图案。与非转基因系相比,ACO活性降至对照的34.48-53.45%,并且乙烯产生不仅在转基因系中降低,但其产生延迟。转基因系具有29-57%的花瓶寿命,而不是非转基因素。

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