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首页> 外文期刊>Acta Horticulturae >Intra- and interspecies variation of terrestrial orchid Spathoglottis species in Peninsular Malaysia using chloroplast DNA
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Intra- and interspecies variation of terrestrial orchid Spathoglottis species in Peninsular Malaysia using chloroplast DNA

机译：使用叶绿体DNA的半岛马来西亚陆地兰花刺肌种子的血液植物刺蝇物种的变异

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摘要

Spathoglottis is a terrestrial orchid that has potential as landscaping plant and commercial value in the floricultural industry. However, orchids have varied morphological characters, which make species identification difficult and are not sufficientfor identification at the species level. Therefore, DNA-based markers are an alternative method used for accurate species identification using chloroplast DNA sequence data. DNA barcoding is a technique for species identification using a short DNA sequence from a standard and agreed-upon position in the genome. Four chloroplast coding regions, matK, rbcL-a, rpoB and rpoCl, were used to differentiate intra- and inter-genetic variation. Eight haplotype groups were identified and form the proposed structure for the DNA barcodes for Spathoglottis species. The combination of rbcL-a, rpoB and rpoCl sequences produced a higher resolution method for the identification and classification between the species. The coding sequences of chloroplast DNA are useful genetic markers for phylogenetic analysis of Spathoglottis at the species level. The phylogenetic analysis clearly revealed that the five Malaysian native species form a similar group, where S. plicata is separate from S. microchilina, S. affinis, S. aurea and S. gracillis.
机译：spathoglottis是一种陆地兰花，具有潜在的景观植物和花卉工业的商业价值。然而，兰花具有不同的形态特征，使物种鉴定困难并且不足以在物种水平上识别。因此，基于DNA的标记是使用叶绿体DNA序列数据的准确物种鉴定的替代方法。 DNA条形码是一种使用短DNA序列的物种鉴定的技术，所述基因组中的标准和达成的位置。使用四个叶绿体编码区，MATK，RBCL-A，RPOB和RPOCL来区分遗传和遗传间变异。鉴定了八个单倍型基团并形成用于母乳素物种的DNA条形码的所提出的结构。 RBCL-A，RPOB和RPOCL序列的组合产生了更高的分辨率方法，用于鉴定和分类。叶绿体DNA的编码序列是用于物种水平的Spathoglotis的系统发育分析的有用遗传标记。系统发育分析清楚地表明，五个马来西亚本地物种形成了类似的组，其中普利特塔与S. Microchilina，S. Affinis，S.Aurea和S. Gracillis分开。

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