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首页> 外文期刊>Acta Horticulturae >The benefit of combining different 7^genes for resistance to tomato leaf curl begomoviruses
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The benefit of combining different 7^genes for resistance to tomato leaf curl begomoviruses

机译:结合不同的7 ^基因的抗性对番茄叶卷曲的抗性的益处

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Leaf curl caused by whitefly-transmitted geminiviruses (begomoviruses) constraints tomato production in many production areas and breeding for durable resistance is an important target. Four of the leaf curl resistance genes [Ty-1/3, Ty-2, ty-5, and ty-6) identified from tomato wild relatives have been extensively studied, but each of these alone generally provides only tolerance to some begomoviruses. Combinations of Ту genes may offer tolerance to a broader range of begomoviruses and increase durability. Marker-assisted selection was used at World Vegetable Center to stack different combinations of these Ту genes into new tomato breeding lines. A set of 15 of these lines carrying different combinations of the Ту genes was screened in the field in Taiwan where Tomato yellow leaf curl Thailand virus (TYLCTHV) and Tomato leaf curl Taiwan virus (ToLCTV) were confirmed as the predominant tomato-infecting begomoviruses, in Thailand where TYLCTHV and Tomato yellow leaf curl Kanchanaburi virus predominated, and in Vietnam where Tomato leaf curl Hainan virus predominated. The same set of lines was also screened in the net-house in the Philippines with an isolate of Tomato leaf curl Philippines virus, and in Vietnam with an isolate of Pepper yellowleaf curl Vietnam virus. The tomato lines reacted slightly differently when challenged with the different begomoviruses in the different countries, though generally, the lines carrying two Ту genes presented milder leaf curl symptoms than those carrying only one Ту gene. However, none of the combinations of Ту genes provided complete resistance (immunity) to any of the challenging viruses.
机译:叶卷曲由粉红色的白细胞(Begomoviruses)(Begomoviruses)约束番茄生产在许多生产区域和耐用性抗性的繁殖是一个重要的目标。从番茄野生亲属中鉴定的四种叶曲线抗性基因[TY-1/3,TY-2,TY-5和TY-6)已被广泛研究,但是这些单独的每种均通常仅为一些原毛病提供耐受性。 ТУ基因的组合可以为更广泛的原缺口病毒提供耐受性,并增加耐用性。在世界蔬菜中心使用标记辅助选择,将这些ТУ基因的不同组合堆叠成新的番茄育种线。在台湾的田地中筛选了一组携带ТУ基因组合的这些线,在台湾的领域中,番茄黄叶卷曲泰国病毒(Tylcthv)和番茄叶卷曲台湾病毒(Tolctv)被证实是主要的番茄感染原野毒病毒,在泰国,Tylcthv和番茄黄叶卷曲北碧病病毒占主导地位,在越南,番茄叶卷曲海南病毒占主导地位。在菲律宾的网站中,菲律宾的网上房屋中也筛选了相同的线条,番茄叶卷曲菲律宾病毒,越南,辣椒黄色卷曲越南病毒的孤立。当诸如不同国家的不同原点潜水病攻击时,番茄线略有不同,但通常,携带两种基因的线呈现比仅携​​带一个ТУ基因的叶片症状更温和的叶片卷曲症状。然而,ТУ基因的组合都没有提供完全的抗性(免疫)给任何挑战病毒。

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