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首页> 外文期刊>Acta Horticulturae >Development of nematode resistant pure pepper lines via anther culture method
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Development of nematode resistant pure pepper lines via anther culture method

机译：通过花药培养方法开发线虫耐纯胡椒线

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This study was carried out in the laboratories and greenhouses of Cukurova University, Alata Horticultural Research Institute and a seed company between 2015 and 2016 to obtain nematode resistant pure pepper lines. The pure lines were obtained from Fiplants via anther culture method. Fi plants were obtained via crossing of three nematode resistant pepper genitors in Fe level (N-269: green -N gen, N-8: green-N gen and N-6: bell-N gen) which were obtained from the studies carried out at the Alata Horticultural Research Institute with breeding pepper lines of a seed company selected in terms of high fruit quality and desired agronomic characters in F3 level (5 green, 5 bell, 5 Charleston and 5 capia). Murashige and Skoog (MS) nutrient medium contained30 g L1 sucrose, 0.25% activated charcoal, 15 mg L1 silver nitrate (AgN03), 4 mg L1 naphthaleneacetic acid (NAA) and 0.5 mg L16-benzylaminopurine (BAP) was used. The cultivated anthers were transferred hormone-free MS nutrient medium after 35 days. As aresult of the study; it was observed that different parameters were changed according to genotypes. The highest embryo yield was obtained from the genotype numbered Z-tad 87 (nematode resistance x bell hybrid). Among crossing between nematode resistanceand different pepper types; bell pepper type gave the highest embryo yield and this was followed by capia, Charleston, and green types. The highest rate of embryos produced plants was observed in green pepper type. The highest haploid plant percentage was observed in capia pepper type and this was followed by Charleston. As a result, all genotypes tested reacted to anther culture and the number of embryo and haploid plants obtained were varied. In the study, three homozygote pure lines contained Mel, Me3, N and Me7 resistance genes were recorded. Also, one homozygote pure lines contained Mel was noted. These nematode resistant genotypes obtained can also be used in breeding studies.
机译：该研究是在Cukurova大学，米塔洛瓦大学的实验室和温室进行，2015年至2016年间，养殖耐纯胡椒线。通过制剂培养方法从氟皿中获得纯线。通过在Fe水平中的三种线性抗性辣椒生殖器中的交叉获得（N-269：Green-N，N-8：Green-n Gen和N-6：Bell-n Gen）而获得，从而获得了所携带的在Alata Hortultululation研究所，在F3水平的高水果质量和期望的农艺人物方面选择了养殖公司的繁殖辣椒系列（5绿色，5钟，5个查尔斯顿和5种Capia）。 Murashige和Skoog（MS）营养介质含有30g L1蔗糖，0.25％活性炭，15mg L1硝酸银（AgN03），4mg L1萘酸（NAA）和0.5mg L16-苄基氨基嘌呤（BAP）。 35天后，培养的花药在35天后转移出自由激素的MS营养培养基。作为研究的遗产;观察到，根据基因型改变不同的参数。从基因型编号的Z-TAD 87（线虫电阻x铃声）获得最高的胚胎产率。线虫抗抗灭虫之间的横穿;甜椒类型给出了最高的胚胎产量，然后是Capia，Charleston和Green类型。在青椒型中观察到胚胎产生的植物的最高速率。在Capia Pepper类型中观察到最高的单倍体植物百分比，然后是Charleston。结果，测试对花药培养的所有基因型和所得胚胎和单倍体植物的数量变化。在该研究中，记录了三种含有MEL，ME3，N和ME7抗性基因的三种纯合理纯线。而且，注意到包含一个纯合纯度含有MEL的纯线。所获得的这些Nematode抗性基因型也可用于育种研究。

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