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首页> 外文期刊>Acta Horticulturae >Identifying self-incompatibility alleles in selected hazelnut genotypes
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Identifying self-incompatibility alleles in selected hazelnut genotypes

机译：识别所选榛子基因型中的自我不相同等位基因

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摘要

Hazelnut industry has newly formed and is developing rapidly in Ontario, Canada, due to the expanding market and public interest for diverse horticultural crops. The hazelnut industry has to face a few challenges to thrive. The major one is the need for new cultivars that are winter hardy and resistant to Eastern Filbert Blight disease, endemic in eastern North America. The next step is to identify the self-incompatibility alleles to ensure fertilization and nut formation. Up to now, about 33 alleleshave been identified in hazelnuts and each genotype carries two alleles. The traditional method to identify the alleles in a genotype is to look at the pollen tube growth of controlled crosses under fluorescent microscopy. This method needs the access to fresh pollen of 33 specific hazelnut genotypes with known 5-alleles as a source of pollen and mature hazelnut tree with unknown 5-alleles as female flowers. The method is also labour intensive and time-sensitive. Several attempts have been made to develop molecular markers to identify 5-alleles in early stages of growth; however, few markers were developed with variable results. The goal of this experiment is to further understand the genetic base of self-incompatibility in hazelnuts. Twenty genotypeswere selected, some with known and the rest with unknown 5-alleles. Female flowers were self-pollinated and RNA from styles was extracted, sequenced and aligned de novo. Primers will be designed based on their homology with s-alleles of brassica and plants with similar incompatibility mechanism. The primers will be validated using DNA of the selected hazelnut genotypes and cultivars.
机译：由于扩大市场和园艺作物的市场和公共利益，榛子产业新成立并在加拿大安大略省开发迅速。榛子产业必须面临一些挑战来茁壮成长。主要是需要新品种，这些品种是冬季耐寒和抵御东部榛树枯萎病，在北美洲的地方性。下一步是识别自我不相容等位基因，以确保施肥和螺母形成。到目前为止，在榛子中鉴定了约33个等位基因，并且每个基因型都有两种等位基因。传统方法以鉴定基因型中的等位基因是看荧光显微镜下受控交叉的花粉管生长。该方法需要获得33种特异性榛子基因型的新鲜花粉，其中包含已知的5型等位基因作为花粉和成熟榛子树的来源，与女性花朵为未知的5个等位基因。该方法也是劳动密集型和时间敏感的。已经进行了几次尝试，以开发分子标记物，以鉴定生长早期阶段的5个等位基因;然而，很少有标记以可变结果开发。该实验的目标是进一步了解榛子中自我不相容性的遗传基因基础。选择了20个基因型，一些有一些已知的，其余的5个等位基因。女性花是自我授粉的，提取来自款式的RNA，测序和对齐de novo。基于它们的同源性与芸薹属的同源物和具有相似不相容机制的植物的同源物设计。使用所选榛子基因型和品种的DNA验证引物。

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  • 来源
    《Acta Horticulturae》 |2019年第1231期|共4页
  • 作者

    T. Taghavi; A. Rahemi; A. Dale; S. Munholland; L.-S. Chia; W. Crosby; J. Kelly;

  • 作者单位

    Department of Plant Agriculture University of Guelph Canada;

    Department of Plant Agriculture University of Guelph Canada;

    Department of Plant Agriculture University of Guelph Canada;

    Department of Plant Agriculture University of Guelph Canada;

    Department of Plant Agriculture University of Guelph Canada;

    Department of Plant Agriculture University of Guelph Canada;

    Department of Plant Agriculture University of Guelph Canada;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 园艺;
  • 关键词

    Corylus; S-genotype; pollen; fertilization; fruit set; style;

    机译：corylus;s-基因型;花粉;施肥;水果套装;风格;

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