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首页> 外文期刊>Acta Horticulturae >Genetic diversity of lycopene epsilon cylase, a carotenoid synthetic gene, among Thai local and commercial papaya cuItivars
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Genetic diversity of lycopene epsilon cylase, a carotenoid synthetic gene, among Thai local and commercial papaya cuItivars

机译：番茄红素ε旋转血糖血清，一种类胡萝卜素合成基因，泰国局部和商业番木瓜中的遗传多样性

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摘要

Carotenoids are groups of tetraterprenoids found naturally contributing to peel and pulp colour in fruits. Carotenoids play important roles in human health including being provitamin A and antioxidants. Papaya (Carica papaya L.) is among fruits with high carotenoid contents with yellow, orange and red flesh colour containing variation of carotenoid molecules. Within carotenoid biosynthetic pathway, conversion of lycopene to alpha carotene by lycopene epsilon cyclase (Lye) is one of the crucial stepsfor regulating carotenoid content. In this study, genetic diversity of Lye was investigated in fifteen papaya samples. Sequence analysis indicated the presence of a single copy of this gene locating in supercontig 28 of papaya genome. Twenty-four pairs of gene specific primers were designed and used to amplify DNA fragment with approximate size of 520-600 base pairs. The results revealed a total of 22 single nucleotide polymorphisms (SNPs) and 3 insertion/deletion. SNPs type C/T had the highest frequency. Almost all of DNA variation was located in intron. One C/A transversion resulted in the conversion of amino acid serine to arginine in exon 1. Phylogenetic analysis indicated that Lye genes from these papaya samples could be divided into two clusters;solo type and Mexico type.
机译：类胡萝卜素是四萜丙丙烯酸戊丙烯酸戊丙酮，发现天然有助于果实剥离和纸浆颜色。类胡萝卜素在人类健康中发挥重要作用，包括禽蛋白A和抗氧化剂。番木瓜（CaricaPapaya L.）是具有高类胡萝卜素内容的水果中，含有黄色，橙色和红色肉颜色，含有类胡萝卜素分子的变化。在类胡萝卜素生物合成途径中，通过番茄红素ε环化酶（LYE）将番茄红素转化为α胡萝卜素（LYE）是调节类胡萝卜素含量的关键步骤之一。在这项研究中，在十五番木瓜样品中研究了Lye的遗传多样性。序列分析表明，在木瓜基因组的超细图28中定位该基因的单一拷贝。设计了二十四对基因特异性引物，并用于扩增具有近似520-600碱基对的DNA片段。结果显示，总共22种单核苷酸多态性（SNP）和3个插入/缺失。 SNP型C / T具有最高频率。几乎所有DNA变异都位于内含子中。一种C / A蒸发导致氨基酸丝氨酸转化为外显子1中的精氨酸1.系统发育分析表明，来自这些木瓜样品的Lye基因可以分为两个簇;单型和墨西哥类型。

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  • 来源
    《Acta Horticulturae》 |2016年第1111期|共6页
  • 作者

    P. Burns; P. Saengmanee; J. Siripanich; T. Chaisan;

  • 作者单位

    Genome Technology Research Unit (GTU) Center for Agricultural Biotechnology Kasetsart University KamphangSaen Campus Center of Excellence on Agricultural Biotechnology (Ag-BIO/PERDO-CHE) Bangkok Thailand;

    Genome Technology Research Unit (GTU) Center for Agricultural Biotechnology Kasetsart University KamphangSaen Campus Center of Excellence on Agricultural Biotechnology (Ag-BIO/PERDO-CHE) Bangkok Thailand;

    Department of Horticulture Faculty of Agriculture at KamphangSaen Kasetsart University KamphangSaen Campus Thailand;

    Department of Agronomy Faculty of Agriculture Kasetsart University Thailand;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 园艺;
  • 关键词

    Carica papaya; colour measurement; phylogenetic tree; genetic diversity; carotenoid biosynthetic pathway;

    机译：Carica Papaya;颜色测量;系统发育树;遗传多样性;类胡萝卜素生物合成途径;

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