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首页> 外文期刊>Acta Horticulturae >Effect of gibberellic acid application on the content of active compounds in leaves and bracts of globe artichoke (Cynara cardunculus var. scolymus L.)
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Effect of gibberellic acid application on the content of active compounds in leaves and bracts of globe artichoke (Cynara cardunculus var. scolymus L.)

机译：甘油酸应用对全球叶片叶片活性化合物含量的影响（Cynara心羽静脉。Scolyymus L.）

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摘要

The artichoke is considered as a "functional food" by containing antioxidant compounds, particularly phenols. The content of these active principles depends on the stage of development, genotype, crop management and the portion of the plant under study. The aim was to determine the concentration of cynarin and chlorogenic acid in leaves and bracts in vegetative and reproductive stages of the plants. The genotypes selected were 'Oro Verde FCA' (OV), 'Guri FCA' (GU) and 'Gauchito FCA' (GA), all of themobtained on the Faculty of Agricultural Sciences, Rosario's National University. The plants were treated with 120 ppm of gibberellic acid (GA3) or untreated. Samples were prepared with 5 leaves of the middle layer of 4 plants in vegetative and reproductive conditions and the bracts of primary heads and stored in freezer (-80°C) until preparation of extracts. Measurements were made at the Mass Spectrometry Laboratory of Scientific and Technology Center (CCT-CONICET Rosario, Argentina). The extract wereanalyzed by HPLC using UV spectroscopy (330 nm) and electrospray MS as detectors. The results showed cynarin (3052 mg kg1) and chlorogenic acid (562 mg kg1) in leaves of GA in vegetative stage without treatment of GA3. The concentration was higher in reproductive conditions (4257 and 1079 mg kg-1, respectively). OV and GU plants in vegetative stage, without treatment of GA3, did not show cynarin in the leaves but they showed chlorogenic acid (863 and 200 mg kg-1, respectively). GA plants treated, in both stages of development, showed lower content of cynarin but it was increased in OV and GU in reproductive stage (540 and 704 mg kg1, respectively). Chlorogenic acid was detected in GU plants treated with GA3 in both stages. Central bracts without GA3 showed chlorogenic acid and cynarin in all genotypes. The application of GA3 showed both active compounds in GU. In the outer bracts, chlorogenic acid not was detected, while cynarin showed higher concentration in GA and OV. Only GU increased the concentration of both active compounds when GA3 was used. These results demonstrate that the genotype and some crop managements can influence the content of active principles.
机译：朝鲜蓟通过含有抗氧化化合物，特别是苯酚被认为是“功能性食物”。这些活性原理的含量取决于开发，基因型，作物管理和研究中的植物部分的阶段。目的是在植物的植物和生殖阶段确定叶片中的枯嗪和绿原酸的浓度。选择的基因型是'ORO Verde FCA'（OV），'Guri FCA'（GU）和'Gauchito FCA'（GA），所有关于农业科学学院的大学，罗萨里奥国立大学。将植物用120ppm的甲苯乙酸（Ga 3）处理或未治疗。制备样品，其中5叶中间层的4种植物中的植物和生殖条件以及初级头部的苞片并储存在冰箱（-80℃）中，直至制备提取物。在科技中心的质谱实验室（CCT-Conicet Rosario，阿根廷）进行了测量。使用UV光谱（330nm）和电喷雾MS作为探测器，通过HPLC展开的提取物。结果在营养期的植物叶片中显示出卵石素（3052mg kg1）和绿原酸（562mg kg1），而不处理Ga3。浓度在繁殖条件下较高（分别为4257和1079mg-1）。 ov和ug植物在营养阶段的植物阶段，没有治疗ga3，在叶子中没有显示叉素，但它们显示出绿色酸（分别为863和200mg-1）。在两个发育阶段治疗的GA植物显示出含量的含量较低，但在ov和生殖阶段（分别为540和704mg kg1）中增加。在两个阶段用GA3处理的ug植物中检测到绿原酸。没有GA3的中央苞片在所有基因型中显示出绿原酸和胞红细胞。 Ga3的施用显示出沟中的活性化合物。在外部苞片中，未检测到绿原酸，而睾丸素在GA和OV中显示出较高的浓度。仅使用GA3时，鉴于沟才增加了两种活性化合物的浓度。这些结果表明，基因型和一些作物管理可以影响活跃原则的内容。

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  • 来源
    《Acta Horticulturae》 |2016年第1147期|共10页
  • 作者

    S.M. Garcia; R. Rotondo; F.S. Lopez Anido; E.L. Cointry; R Santa Cruz; R. Furlan; A.M. Escalante;

  • 作者单位

    Catedra Sistemas de Cultivos Intensivos-Area Horticultura Facultad de Ciencias Agrarias UNR Argentina;

    Catedra Sistemas de Cultivos Intensivos-Area Horticultura Facultad de Ciencias Agrarias UNR Argentina;

    Catedra de Genetica Facultad de Ciencias Agrarias UNR Argentina;

    Catedra de Mejoramiento Vegetal Facultad de Ciencias Agrarias UNR Argentina;

    lnstituto de Investigaciones para el Descubrimiento de Farmacos de Rosario UNR-CONICET Argentina;

    lnstituto de Investigaciones para el Descubrimiento de Farmacos de Rosario UNR-CONICET Argentina;

    lnstituto de Investigaciones para el Descubrimiento de Farmacos de Rosario UNR-CONICET Argentina;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 园艺;
  • 关键词

    cynarin; chlorogenic acid; antioxidant; HPLC-UV-MS;

    机译：cynarin;绿原酸;抗氧化剂;HPLC-UV-MS;

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