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首页> 外文期刊>Acta Horticulturae >Innovative Fi hybrid method enables testing of large number of line to line crossings for combining ability
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Innovative Fi hybrid method enables testing of large number of line to line crossings for combining ability

机译：创新的FI混合方法可以测试大量线路到线路交叉以组合能力

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摘要

Hybrid vigor has been demonstrated in the early 20th century after hybrid corn was invented. Procedures to breed hybrid cultivar are more complex than breeding open-pollinated cultivars or pure lines. Hybrid cultivars are most often based on the crossing of two true breeding lines that should genetically complement each other. This complementarity of genotypes of genetically different parental lines in the Fi hybrid often results in a considerable improvement of e.g. growth characteristics, yield oradaptation to environmental stresses as compared to the individual parental lines and non-hybrid cultivars. Numerous methods have been proposed, the most common is based on development of large numbers of inbred lines originated form heterozygous parentsby either self pollination or doubled haploid induction. Inbred lines are then tested for hybrid vigor often in two steps, by which first "general combining ability" and then "specific combining ability" is tested to identify a pair that expresses optimal characteristics. This standard procedure allows only a very limited number of line to line crosses since it is laborious and long lasting. Here we are proposing a new method that allows much higher number of line to line crosses as previously known. Our invention relates to innovative method of testing combining ability of inbred lines, which is based on genotyping of each genetically diverse inbred line followed by allowing the plants of the donor lines to intercross to obtain Fi hybrid progeny. Inthe next season progeny is phenotyped on individual basis. For superior individuals parental lines are revealed by paternity testing, which due to homozygosity of lines and previous genotyping allows identifying both parental lines. By this method a muchlarger number of line to line testing can be achieved thus overcoming major bottleneck in breeding hybrid cultivars. Details of the protocol and its utilization will be discussed.
机译：在20世纪初期发明后，已经证明了混合活力。繁殖杂种品种的程序比繁殖开放授粉品种或纯线更复杂。杂交品种通常是基于两条真正的育种线的交叉，这些系列应该彼此依法补充。这种杂交中的遗传学不同亲本系基因型的这种互补性往往导致例如相当大的改善。与个体父母系和非杂交品种相比，生长特征，对环境应激的产量或环境胁迫。已经提出了许多方法，最常见的是基于大量自交线的发展源自自授粉或双倍单倍体诱导的杂授予的杂种父母。然后经常以两个步骤进行混合活力的近交线，首先测试第一“一般组合能力”，然后测试“特定的组合能力”以识别表达最佳特性的对。此标准程序仅允许非常有限的线路交叉线，因为它是费力和持久的持久性。在这里，我们提出了一种新方法，其允许更高数量的线路交叉，如前所述。我们的发明涉及测试近交系的结合能力的创新方法，这是基于每个遗传多种自交线的基因分型，然后允许供体系的植物进行杂交后代。下一个季节后代是在个人基础上表现出来的。对于优质的个体，父母线被父族试验揭示，这是由于线的纯合子和先前的基因分型允许识别父母。通过该方法，可以实现更加多Llarger的线路测试线，因此克服繁殖杂种品种的主要瓶颈。将讨论协议的详细信息及其利用率。

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