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Seeding Protein Crystallization with Cross-Linked Protein Crystals

机译：用交联蛋白质结晶播种蛋白质结晶

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摘要

Protein crystallization is of great importance because protein crystals have a number of different important applications, including large-scale purification of proteins, determination of protein structure, nanoparticle preparation, and theoretical studies of crystallization. An approach often used to efficiently crystallize proteins is the use of nucleants or seeds (small fragments of protein crystals) that can help increase the probability of protein crystallization. Due to the very positive effect that seeding has on protein crystallization, seeds are now widely accepted and utilized in practical protein crystallization. Here, we show that cross-linked protein crystals (CLPCs), which retain the crystal structure but are much more stable than non-cross-linked crystals, can also be used as a new type of seed for promoting protein crystallization. Seeding with CLPCs has effects on both the reproducibility and screening of protein crystals and could improve the optical perfection (well-defined facets) of protein crystals and the probability of obtaining protein crystals. In addition, the cross-linked protein crystals may reduce the concentration of protein molecules needed to obtain protein crystals. Furthermore, CLPCs are very stable in air, and no protective medium is necessary for the long-term storage of CLPCs. This feature makes the CLPC seeding method a potentially powerful technique in practical protein crystallization on either a laboratorial or an industrial scale.
机译：蛋白质结晶是非常重要的，因为蛋白质晶体具有许多不同的重要应用，包括蛋白质大规模纯化，蛋白质结构的测定，结晶的理论研究和结晶的理论研究。通常用于有效地结晶蛋白质的方法是使用核酸或种子（蛋白质晶体的小片段），这有助于增加蛋白质结晶的概率。由于播种对蛋白质结晶的非常积极的影响，现在广泛接受并在实际蛋白质结晶中得到种子。这里，我们表明交联蛋白质晶体（CLPC），其保留晶体结构但比非交联晶体更稳定，也可以用作用于促进蛋白质结晶的新型种子。播种CLPCS对蛋白质晶体的再现性和筛选具有影响，并且可以改善蛋白质晶体的光学完美（明确刻面）和获得蛋白质晶体的可能性。另外，交联蛋白质晶体可以减少获得蛋白质晶体所需的蛋白质分子的浓度。此外，CLPC在空气中非常稳定，并且对CLPC的长期储存没有保护介质。该特征使CLPC播种方法在实际蛋白质结晶中具有实验室或工业规模的潜在强大的技术。

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  • 来源
    《Crystal growth & design》 |2018年第2期|共11页
  • 作者

    Er-Kai Yan; Feng-Zhu Zhao; Chen-Yan Zhang; Xue-Zhou Yang; Miao Shi; Jin He; Ya-Li Liu; Yue Liu; Hai Hou; Da-Chuan Yin;

  • 作者单位

    Institute for Special Environmental Biophysics Key Laboratory for Space Bioscience and Space Biotechnology School of Life Sciences Northwestern Polytechnical University Xi’an 710072 Shaanxi People’s Republic of China;

    Institute for Special Environmental Biophysics Key Laboratory for Space Bioscience and Space Biotechnology School of Life Sciences Northwestern Polytechnical University Xi’an 710072 Shaanxi People’s Republic of China;

    Institute for Special Environmental Biophysics Key Laboratory for Space Bioscience and Space Biotechnology School of Life Sciences Northwestern Polytechnical University Xi’an 710072 Shaanxi People’s Republic of China;

    Institute for Special Environmental Biophysics Key Laboratory for Space Bioscience and Space Biotechnology School of Life Sciences Northwestern Polytechnical University Xi’an 710072 Shaanxi People’s Republic of China;

    Institute for Special Environmental Biophysics Key Laboratory for Space Bioscience and Space Biotechnology School of Life Sciences Northwestern Polytechnical University Xi’an 710072 Shaanxi People’s Republic of China;

    Institute for Special Environmental Biophysics Key Laboratory for Space Bioscience and Space Biotechnology School of Life Sciences Northwestern Polytechnical University Xi’an 710072 Shaanxi People’s Republic of China;

    Institute for Special Environmental Biophysics Key Laboratory for Space Bioscience and Space Biotechnology School of Life Sciences Northwestern Polytechnical University Xi’an 710072 Shaanxi People’s Republic of China;

    Institute for Special Environmental Biophysics Key Laboratory for Space Bioscience and Space Biotechnology School of Life Sciences Northwestern Polytechnical University Xi’an 710072 Shaanxi People’s Republic of China;

    Institute for Special Environmental Biophysics Key Laboratory for Space Bioscience and Space Biotechnology School of Life Sciences Northwestern Polytechnical University Xi’an 710072 Shaanxi People’s Republic of China;

    Institute for Special Environmental Biophysics Key Laboratory for Space Bioscience and Space Biotechnology School of Life Sciences Northwestern Polytechnical University Xi’an 710072 Shaanxi People’s Republic of China;

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  • 正文语种 eng
  • 中图分类 晶体学;
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