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Direct Crystallization of Proteins from Impure Sources

机译：从纯根中直接结晶蛋白质

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摘要

In recent years, with the rapidly increasing demand for pure protein products in various fields (biomedicines, biochemical reagents, food industries, etc.), the need for low-cost, high-quality protein purification technology has become urgent. Under this background, the traditional purification technology, protein crystallization, comes back to people's attention. Protein crystallization has the ability to obtain high-quality protein products at a low cost. Nevertheless, protein crystallization itself is challenging; for a long time, the industrial purification of proteins often has used chromatography-based approaches. In the field of structural biology, the strong demand for protein crystals has led to the full development of protein crystallization technology. To date, these technologies may have the potential to provide solutions to achieve crystallization of proteins at the industrial scale. In this paper, we report our effort to screen the crystallization conditions of four sample proteins (lysozyme, hemoglobin, superoxide dismutase, and homoserine oxygen-acetyltransferase) from different impure sources (natural and recombined ones). It was confirmed that crystallization screening technology allows protein crystals to be obtained directly from impure protein sources, showing that, from the impure sources, the target protein can be purified directly by crystallization without prior purification using chromatographic processes. This work demonstrated that the new technologies developed in the field of protein crystallization methodologies can be well applied in solving problems in traditional purification technology.
机译：近年来，随着对各个领域的纯蛋白质产品的需求迅速增加（生物医药，生物化学试剂，食品工业等），需要低成本，高质量的蛋白质净化技术已成为迫切。在这种背景下，传统的净化技术，蛋白质结晶，恢复了人们的注意。蛋白质结晶具有以低成本获得高质量蛋白质产品的能力。然而，蛋白质结晶本身是挑战性的;长期以来，蛋白质的工业纯化通常使用基于色谱的方法。在结构生物学领域中，对蛋白质晶体的强烈需求导致了蛋白质结晶技术的全面发展。迄今为止，这些技术可能有可能提供解决方案以在工业规模中实现蛋白质的结晶。在本文中，我们向我们努力筛选来自不同纯度（天然和重组）的四种样品蛋白（溶菌酶，血红蛋白，超氧化物歧化酶和均静氧氧 - 乙酰转移酶）的结晶条件。证实结晶筛选技术允许直接从不纯的蛋白质来源获得蛋白质晶体，表明，从不太源，可以通过结晶直接纯化靶蛋白，而无需使用色谱法纯化。这项工作表明，在蛋白质结晶方法领域开发的新技术可以很好地应用于传统净化技术中的问题。

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  • 来源
    《Crystal growth & design》 |2020年第3期|共12页
  • 作者

    Liu Yue; Hou Hai; Li Jin; Cheng Qing-Di; Zhang Xi; Zeng Xiang-Bin; Fiaz Ahmad; Wang Bo; Zhang Chen-Yan; Lu Qin-Qin; Yin Da-Chuan;

  • 作者单位

    Northwestern Polytech Univ Sch Life Sci Key Lab Space Biosci &

    Biotechnol Inst Special Environm Biophys Xian 710072 Shaanxi Peoples R China;

    Northwestern Polytech Univ Sch Life Sci Key Lab Space Biosci &

    Biotechnol Inst Special Environm Biophys Xian 710072 Shaanxi Peoples R China;

    Northwestern Polytech Univ Sch Life Sci Key Lab Space Biosci &

    Biotechnol Inst Special Environm Biophys Xian 710072 Shaanxi Peoples R China;

    Northwestern Polytech Univ Sch Life Sci Key Lab Space Biosci &

    Biotechnol Inst Special Environm Biophys Xian 710072 Shaanxi Peoples R China;

    Northwestern Polytech Univ Sch Life Sci Key Lab Space Biosci &

    Biotechnol Inst Special Environm Biophys Xian 710072 Shaanxi Peoples R China;

    Northwestern Polytech Univ Sch Life Sci Key Lab Space Biosci &

    Biotechnol Inst Special Environm Biophys Xian 710072 Shaanxi Peoples R China;

    Northwestern Polytech Univ Sch Life Sci Key Lab Space Biosci &

    Biotechnol Inst Special Environm Biophys Xian 710072 Shaanxi Peoples R China;

    Northwestern Polytech Univ Sch Life Sci Key Lab Space Biosci &

    Biotechnol Inst Special Environm Biophys Xian 710072 Shaanxi Peoples R China;

    Northwestern Polytech Univ Sch Life Sci Key Lab Space Biosci &

    Biotechnol Inst Special Environm Biophys Xian 710072 Shaanxi Peoples R China;

    Northwestern Polytech Univ Sch Life Sci Key Lab Space Biosci &

    Biotechnol Inst Special Environm Biophys Xian 710072 Shaanxi Peoples R China;

    Northwestern Polytech Univ Sch Life Sci Key Lab Space Biosci &

    Biotechnol Inst Special Environm Biophys Xian 710072 Shaanxi Peoples R China;

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  • 正文语种 eng
  • 中图分类 晶体学;
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