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首页> 外文期刊>Crystal growth & design >Continuous Crystallization of Proteins in a Stirred Classified Product Removal Tank with a Tubular Reactor in Bypass
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Continuous Crystallization of Proteins in a Stirred Classified Product Removal Tank with a Tubular Reactor in Bypass

机译：旁路管式反应器中搅拌分类产物去除罐中蛋白质的连续结晶

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摘要

A lab-scale stirred tank with a cooled tubular reactor in bypass was applied for continuous crystallization of lysozyme and a full-length therapeutic monoclonal antibody. The stirred tank was operated as a mixed suspension classified product removal crystallizer. Lysozyme was crystallized by a combination of cooling crystallization and salting-out. The antibody was crystallized by a combination of cooling crystallization and isoelectric crystallization. It was deduced that nucleation rates were enhanced when the protein solutions passed through the cooled tubular bypass. It was further deduced that crystal growth rates were enhanced in the stirred tank which was operated at a higher temperature compared to the tubular reactor. Classified product removal was possible by controlled sedimentation of protein crystals. The continuous crystallization system allowed a targeted control of crystal morphology and size. No sedimentation occurred in the tubular reactor and precipitation was avoided at all times. High crystallization yields of more than 90% were obtained. Crystals of the monoclonal antibody were continuously produced for the first time with a space-time yield of up to 12 g L-1 h(-1).
机译：施加具有冷却管式反应器的实验室搅拌槽，用于连续结晶溶菌酶和全长治疗单克隆抗体。将搅拌的罐作为混合悬浮液分类产品去除结晶器操作。通过冷却结晶和盐洗的组合结晶溶菌酶。通过冷却结晶和等电结晶的组合结晶抗体。推导出在通过冷却的管状旁路通过时，提高了成核率。进一步推导出在与管状反应器相比在较高温度下在较高温度下操作的搅拌罐中的晶体生长速率增强。通过控制蛋白质晶体的沉淀可以进行分类产品去除。连续结晶系统允许拟晶体形态和尺寸的靶向控制。管状反应器中没有发生沉降，始终避免沉淀。获得高于90％以上的高结晶产率。连续使用单克隆抗体的晶体首次产生高达12g L-1 H（-1）的时空产率。

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  • 来源
    《Crystal growth & design》 |2017年第8期|共8页
  • 作者

    Hekmat Dariusch; Huber Max; Lohse Christian; von den Eichen Nikolas; Weuster-Botz Dirk;

  • 作者单位

    Tech Univ Munich Inst Biochem Engn Boltzmannstr 15 D-85748 Garching Germany;

    Tech Univ Munich Inst Biochem Engn Boltzmannstr 15 D-85748 Garching Germany;

    Tech Univ Munich Inst Biochem Engn Boltzmannstr 15 D-85748 Garching Germany;

    Tech Univ Munich Inst Biochem Engn Boltzmannstr 15 D-85748 Garching Germany;

    Tech Univ Munich Inst Biochem Engn Boltzmannstr 15 D-85748 Garching Germany;

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  • 正文语种 eng
  • 中图分类 晶体学;
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