...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>ACS applied materials & interfaces >Rapid Buildup Arrays with Orthogonal Biochemistry Gradients via Light-Induced Thiol-Ene 'Click' Chemistry for High-Throughput Screening of Peptide Combinations
【24h】

Rapid Buildup Arrays with Orthogonal Biochemistry Gradients via Light-Induced Thiol-Ene 'Click' Chemistry for High-Throughput Screening of Peptide Combinations

机译：快速积聚阵列，具有正交生物化学梯度通过光诱导的硫醇-NEE“点击”化学以获得肽组合的高通量筛选

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The concept of high-throughput screening sheds new light on fabrication and analysis of materials. Herein, a combinatorial surface-modified platform with biochemical gradients was developed through thiol-ene "click" chemistry by adjusting the intensity of ultraviolet (UV) irradiation. Contact angle, X-ray photoelectron spectroscopy, and ellipsometry measurement results demonstrated that the sulfhydryl molecules including polyethylene glycol and RGD (arginine-glycine-aspartic acid) and REDV (arginine-glutamic acid- aspartic acid-valine) peptides can be directly attached onto alkene-modified substrates, in which the graft density can be well controlled by the intensity of UV irradiation. The multistep attachment of different molecules onto substrates is archived via the multistep UV-initiated thiol-ene "click" reaction. The high-throughput arrays with the gradient density of single ligand and the orthogonal gradient density of two ligands were rapidly fabricated via the one-step UV gradient irradiation and the two-step orthogonal UV gradient-initiated thiol-ene "click" reaction. Endothelial cells (ECs) and smooth muscle cells (SMCs) were cocultured on the array with the orthogonal gradient density of RGD and REDV to screen the peptide combination with high EC selectivity, which is essential for in situ endothelialization during stent implant. From 64, 8 X 8, combinations investigated, a special combinatorial surface representing the really high competitiveness of ECs over SMCs was screened. This platform puts forward a facile, high-throughput method to study the combinatorial variation of biochemical signals to cell behavior.
机译：高吞吐量筛选揭示新灯的制造和材料分析。这里，通过调节紫外（UV）照射的强度，通过硫醇-Ne“点击”化学产生具有生化梯度的组合表面改性平台。接触角，X射线光电子能谱和椭圆形测量结果证明，包括聚乙二醇和RGD（精氨酸 - 甘氨酸 - 天冬氨酸）和REDV（精氨酸 - 谷氨酸 - 天冬氨酸 - 缬氨酸）肽等巯基分子可以直接连接到烯烃改性衬底，其中移植物密度可以通过UV辐射的强度良好控制。将不同分子的MultiSep附着在基材上通过MultiSep UV引发的硫醇-ne“点击”反应来归档。通过单步UV梯度照射和两步正交紫外梯度引发的硫醇-eNE“点击”反应，通过单步UV梯度辐射快速制造具有单一配体的梯度密度的高通量阵列和两个配体的正交梯度密度。内皮细胞（ECS）和平滑肌细胞（SMC）以RGD和REDV的正交梯度密度与RGD和REDV的正交梯度密度筛选，以筛选具有高EC选择性的肽组合，这对于支架植入物期间的原位内皮是必不可少的。从64,8 x 8，调查的组合，筛选了一个特殊的组合表面，代表了SMCs上的ECS真正高竞争力的组合表面。该平台提出了一种容易，高通量的方法来研究生物化学信号的组合变化到细胞行为。

著录项

  • 来源
    《ACS applied materials & interfaces》 |2020年第18期|共10页
  • 作者

    Hao Hongye; Huang Junjie; Liu Ping; Xue Yunfan; Wang Jing; Jia Fan; Ren Kefeng; Jin Qiao; Ji Jian;

  • 作者单位

    Zhejiang Univ Dept Polymer Sci &

    Engn MOE Key Lab Macromol Synth &

    Functionalizat Hangzhou 310027 Peoples R China;

    Zhejiang Univ Dept Polymer Sci &

    Engn MOE Key Lab Macromol Synth &

    Functionalizat Hangzhou 310027 Peoples R China;

    Zhejiang Univ Dept Polymer Sci &

    Engn MOE Key Lab Macromol Synth &

    Functionalizat Hangzhou 310027 Peoples R China;

    Zhejiang Univ Dept Polymer Sci &

    Engn MOE Key Lab Macromol Synth &

    Functionalizat Hangzhou 310027 Peoples R China;

    Zhejiang Univ Dept Polymer Sci &

    Engn MOE Key Lab Macromol Synth &

    Functionalizat Hangzhou 310027 Peoples R China;

    Zhejiang Univ Dept Polymer Sci &

    Engn MOE Key Lab Macromol Synth &

    Functionalizat Hangzhou 310027 Peoples R China;

    Zhejiang Univ Dept Polymer Sci &

    Engn MOE Key Lab Macromol Synth &

    Functionalizat Hangzhou 310027 Peoples R China;

    Zhejiang Univ Dept Polymer Sci &

    Engn MOE Key Lab Macromol Synth &

    Functionalizat Hangzhou 310027 Peoples R China;

    Zhejiang Univ Dept Polymer Sci &

    Engn MOE Key Lab Macromol Synth &

    Functionalizat Hangzhou 310027 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学工业;
  • 关键词

    high-throughput screening; surface modification; orthogonal gradients; cell adhesion; endothelial cells; smooth muscle cells; competitiveness;

    机译：高通量筛选;表面改性;正交梯度;细胞粘附;内皮细胞;平滑肌细胞;竞争力;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号