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首页> 外文期刊>ACS applied materials & interfaces >Lipase Immobilized on a Novel Rigid-Flexible Dendrimer-Grafted Hierarchically Porous Magnetic Microspheres for Effective Resolution of (R,S)-1-Phenylethanol
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Lipase Immobilized on a Novel Rigid-Flexible Dendrimer-Grafted Hierarchically Porous Magnetic Microspheres for Effective Resolution of (R,S)-1-Phenylethanol

机译：固定在新的刚性柔性树枝状聚合物接枝的分层多孔磁性微球上固定的脂肪酶，用于（R，S）-1-苯基乙醇的有效分辨率

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With the rapid development of biotechnological industry, there is an urgent need for exploiting new materials to immobilize enzymes to improve the performance of biocatalysts. In this paper, hierarchically porous magnetic microspheres (PFMMs) were prepared through solvothermal method and rapidly grafted with a novel rigid-flexible dendrimer first synthesized from monomers of trimesoyl chloride (TMC) and 1,6-hexanediamine (HDA) via interfacial polymerization process for covalent immobilization of Pseudomonas fluorescens lipase (PFL). The maximum PFL loading of the synthesized support reaches 87.5 Mg-protein/g(support), and 864% activity recovery of PFMMs-G(3.0)PFL can be achieved at pH 9.0. Then, it was used to catalyze the resolution of (R,S)-1-phenylethanol with vinyl acetate. Under the optimized conditions, 50.0% conversion with 99.0% ee(s )can be reached within 1.5 h. In addition, a conversion of 49.2% and ee s of 96.9% can be retained after 10 batches of running, displaying an excellent operational stability. Importantly, a further investigation shows that the obviously improved reusability of the immobilized PFL is ascribed to the increased rigidity in comparison to fully flexible dendrimer. Thus, the newly constructed protocol for lipase immobilization exhibits a great prospect in biochemical engineering.
机译：随着生物技术行业的快速发展，迫切需要利用新材料来固定酶以改善生物催化剂的性能。在本文中，通过溶剂质方法制备分层多孔磁性微球（PFMMS），并通过界面聚合方法将首先用三咪酰氯（TMC）和1,6-己二胺（HDA）的单体合成的新型刚性柔性树枝状聚合物。荧光荧光酶（PFL）的共价固定化。合成载体的最大PFL负载达到87.5mg-蛋白/ g（载体），并且可以在pH 9.0下实现PFMMS-G（3.0）PFL的864％活性回收。然后，它用于催化与乙酸乙烯酯的（R，S）-1-苯基乙醇的分辨率。在优化的条件下，50.0％的转化率可以在1.5小时内达到99.0％EE。此外，在10批运行后，可以保留49.2％，EE S的转化率为96.9％，显示出优异的操作稳定性。重要的是，进一步的研究表明，与完全柔性的树枝状聚合物相比，固定的PFL的可重复可重用性归因于增加的刚性。因此，用于脂肪酶固定化的新构建的方案在生化工程中表现出巨大的前景。

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