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首页> 外文期刊>ACS applied materials & interfaces >Direct Determination of Redox Statuses in Biological Thiols and Disulfides with Noncovalent Interactions of Poly(ionic liquid)s
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Direct Determination of Redox Statuses in Biological Thiols and Disulfides with Noncovalent Interactions of Poly(ionic liquid)s

机译：具有聚（离子液体）的非共价相互作用的生物硫醇和二硫化中的氧化还原状态的直接测定

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摘要

The three most important redox couples, including cysteine (Cys)/cystine (Cyss), homocysteine (Hcys)/homocystine (Hcyss), and reduced glutathione (GSH)/glutathione disulfide (GSSG), are closely associated with human aging and many diseases. Thus, it is highly important to determine their redox statuses at the following two levels: (i) the redox identity in different thiols/disulfides and (ii) the redox ratio in a mixture of a specific couple. Herein, by using one single AIE-doped (AIE, aggregation-induced emission) photonic-structured poly(ionic liquid) (PIL) sphere as a virtual sensor array, we realize a direct determination of the redox status without a reducing pretreatment of disulfides, which will greatly promote the development of high-throughput and simple procedures. The pattern-recognition method uses the multiple noncovalent interactions of imidazolium-based PILs with these redox species to produce differential responses in both the photonic crystal and fluorescence dual channels. On the one hand, a single sphere enables the direct and simultaneous discrimination of the redox identities of Cys, Cyss, Hcys, Hcyss, GSH, and GSSG under the interference of other five commonly occurring thiols. On the other hand, this sphere also allows for not only a direct quantification of the GSH/GSSG ratios without previously determining the individual concentrations of GSH and GSSG but also the accurate prediction of the ratios in unknown redox samples. To further demonstrate applications of this method, redox mixtures in a biological sample are differentiated. Additionally, quantum calculations further support our assignments for interactions between the imidazolium-based PILs and these redox species.
机译：三个最重要的氧化还原伴侣，包括半胱氨酸（Cys）/胱氨酸（Cyss），同型酮（HCYS）/同型（HCYSS）和还原的谷胱甘肽（GSH）/谷胱甘肽二硫化物（GSSG）与人类衰老和许多疾病密切相关。因此，在以下两级确定其氧化还原状态非常重要：（i）不同硫醇/二硫化物中的氧化还原性和（ii）特定夫妇的混合物中的氧化还原比。在此，通过使用一个单一AIE掺杂（AIE，聚集诱导的发射）光子结构聚（离子液体）（IONIC液体）球体作为虚拟传感器阵列，我们实现了直接测定氧化还原状态而不降低二硫化物的预处理，这将大大促进高吞吐量和简单的程序的发展。图案识别方法使用咪唑鎓基堆的多个非共价相互作用与这些氧化还原物种，以在光子晶体和荧光双通道中产生差分响应。一方面，单个球体能够在其他五个常见的硫醇的干扰下直接和同时辨别Cys，Cyss，Hcys，Hcyss，GSH和GSSG的氧化还原身份。另一方面，该球体还允许不仅可以直接定量GSH / GSSG比率，而无需先前确定GSH和GSSG的个体浓度，而且还允许GSH和GSSG的单独浓度，而且还允许在未知的氧化还原样品中的比率预测。为了进一步证明该方法的应用，将生物样品中的氧化还原混合物分化。另外，量子计算进一步支持我们的咪唑鎓碱度和这些氧化还原物种之间的相互作用的作用。

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  • 来源
    《ACS applied materials & interfaces》 |2019年第33期|共9页
  • 作者

    Zhang Wanlin; Li Yao; Liang Yun; Yin Xianpeng; Liu Chengcheng; Wang Shigiang; Tian Li; Dong Hao; Li Guangtao;

  • 作者单位

    Tsinghua Univ Minist Educ Key Lab Organ Optoelect &

    Mol Engn Dept Chem Beijing 100084 Peoples R China;

    Chinese Acad Sci Inst Proc Engn Beijing 100190 Peoples R China;

    Tsinghua Univ Minist Educ Key Lab Organ Optoelect &

    Mol Engn Dept Chem Beijing 100084 Peoples R China;

    Aerosp Res Inst Special Mat &

    Proc Technol Beijing 100074 Peoples R China;

    Tsinghua Univ Minist Educ Key Lab Organ Optoelect &

    Mol Engn Dept Chem Beijing 100084 Peoples R China;

    Tsinghua Univ Minist Educ Key Lab Organ Optoelect &

    Mol Engn Dept Chem Beijing 100084 Peoples R China;

    Tsinghua Univ Minist Educ Key Lab Organ Optoelect &

    Mol Engn Dept Chem Beijing 100084 Peoples R China;

    Tsinghua Univ Minist Educ Key Lab Organ Optoelect &

    Mol Engn Dept Chem Beijing 100084 Peoples R China;

    Tsinghua Univ Minist Educ Key Lab Organ Optoelect &

    Mol Engn Dept Chem Beijing 100084 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学工业;
  • 关键词

    biothiol; redox status; sensor array; poly(ionic liquid); photonic crystal;

    机译：Biothiol;氧化还原状态;传感器阵列;聚（离子液体）;光子晶体;

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