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首页> 外文期刊>ACS applied materials & interfaces >Specific and Quantitative Detection of Albumin in Biological Fluids by Tetrazolate-Functionalized Water-Soluble AIEgens
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Specific and Quantitative Detection of Albumin in Biological Fluids by Tetrazolate-Functionalized Water-Soluble AIEgens

机译：四唑官能化水溶性抗血糖用生物液中白蛋白的特异性和定量检测

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摘要

The analysis of albumin has clinical significance in diagnostic tests and obvious value to research studies on the albumin-mediated drug delivery and therapeutics. The present immunoassay, instrumental techniques, and colorimetric methods for albumin detection are either expensive, troublesome, or insensitive. Herein, a class of water-soluble tetrazolate-functionalized derivatives with aggregation-induced emission (AIE) characteristics is introduced as novel fluorescent probes for albumin detection. They can be selectively lighted up by site-specific binding with albumin. The resulting albumin fluorescent assay exhibits a low detection limit (0.21 nM), high robustness in aqueous buffer (pH = 6-9), and a broad tunable linear dynamic range (0.02-3000 mg/L) for quantification. The tetrazolate functionality endows the probes with a superior water solubility (>0.01 M) and a high binding affinity to albumin (K-D = 0.25 mu M). To explore the detection mechanism, three unique polar binding sites on albumin are computationally identified, where the multivalent tetrazolate-lysine interactions contribute to the tight binding and restriction of the molecular motion of the ATE probes. The key role of lysine residues is verified by the detection of poly-L-lysine. Moreover, we applied the fluorogenic method to quantify urinary albumin in clinical samples and found it a feasible and practical strategy for albumin analysis in complex biological fluids.
机译：白蛋白分析对白蛋白介导的药物递送和治疗剂研究研究的临床意义。本发明的免疫测定，仪器技术和比色方法是昂贵的，麻烦或不敏感的。在此，将一类具有聚集诱导的发射（AIE）特征的水溶性四唑酯官能化衍生物作为白蛋白检测的新型荧光探针。它们可以通过与白蛋白的特异性结合选择性地点亮。所得白蛋白荧光测定表现出低检测极限（0.21nm），水缓冲液（pH = 6-9）的高稳健性，以及用于定量的宽可调线性动态范围（0.02-3000mg / L）。四唑酯功能赋予探针具有优异的水溶性（> 0.01M）和对白蛋白的高结合亲和力（K-D =0.25μm）。为了探索检测机制，在计算上鉴定白蛋白的三个独特的极性结合位点，其中多价四唑酸酯 - 赖氨酸相互作用有助于吃得探针的分子运动的紧密结合和限制。通过检测聚L-赖氨酸验证赖氨酸残基的关键作用。此外，我们应用荧光法在临床样品中量化尿白蛋白，并发现复杂生物流体中白蛋白分析的可行性和实用性策略。

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  • 来源
    《ACS applied materials & interfaces》 |2019年第33期|共11页
  • 作者

    Tu Yujie; Yu Yeqing; Zhou Zhibiao; Sheng Xie; Yao Bicheng; Guan Shujuan; Bo Situ; Yong Liu; Kwok Ryan T. K.; Lam Jacky W. Y.; Chen Sijie; Huang Xuhui; Zeng Zebing; Tang Ben Zhong;

  • 作者单位

    Hunan Univ Coll Chem &

    Chem Engn State Key Lab Chemo Biosensing &

    Chemometr Changsha 410082 Hunan Peoples R China;

    Hong Kong Univ Sci &

    Technol Dept Chem Kowloon Clear Water Bay Hong Kong 999077 Peoples R China;

    Hunan Univ Coll Chem &

    Chem Engn State Key Lab Chemo Biosensing &

    Chemometr Changsha 410082 Hunan Peoples R China;

    Hunan Univ Coll Chem &

    Chem Engn State Key Lab Chemo Biosensing &

    Chemometr Changsha 410082 Hunan Peoples R China;

    Hong Kong Univ Sci &

    Technol Dept Chem Kowloon Clear Water Bay Hong Kong 999077 Peoples R China;

    Southern Med Univ Nanfang Hosp Dept Lab Med Guangzhou 510515 Guangdong Peoples R China;

    Southern Med Univ Nanfang Hosp Dept Lab Med Guangzhou 510515 Guangdong Peoples R China;

    Hong Kong Univ Sci &

    Technol Dept Chem Kowloon Clear Water Bay Hong Kong 999077 Peoples R China;

    Hong Kong Univ Sci &

    Technol Dept Chem Kowloon Clear Water Bay Hong Kong 999077 Peoples R China;

    Hong Kong Univ Sci &

    Technol Dept Chem Kowloon Clear Water Bay Hong Kong 999077 Peoples R China;

    Karolinska Inst Ming Wai Lau Ctr Reparat Med Hong Kong 999077 Peoples R China;

    Hong Kong Univ Sci &

    Technol Dept Chem Kowloon Clear Water Bay Hong Kong 999077 Peoples R China;

    Hunan Univ Coll Chem &

    Chem Engn State Key Lab Chemo Biosensing &

    Chemometr Changsha 410082 Hunan Peoples R China;

    Hunan Univ Coll Chem &

    Chem Engn State Key Lab Chemo Biosensing &

    Chemometr Changsha 410082 Hunan Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学工业;
  • 关键词

    albumin; aggregation-induced emission; water-soluble fluorescent probe; tetrazolate-lysine interaction; diagnostic detection;

    机译：白蛋白;聚集诱导的发射;水溶性荧光探针;四唑酸酯 - 赖氨酸相互作用;诊断检测;

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