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首页> 外文期刊>ACS applied materials & interfaces >Hydrogel Bioink with Multilayered Interfaces Improves Dispersibility of Encapsulated Cells in Extrusion Bioprinting
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Hydrogel Bioink with Multilayered Interfaces Improves Dispersibility of Encapsulated Cells in Extrusion Bioprinting

机译：具有多层界面的水凝胶生物孔可提高挤出生物印刷中包封细胞的分散性

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摘要

One of the challenges for extrusion bioprinting using low-viscosity bioinks is the fast gravity-driven sedimentation of cells. Cells in a hydrogel bioink that features low viscosity tend to settle to the bottom of the bioink reservoir, and as such, their bioprintability is hindered by association with the inhomogeneous cellularized structures that are deposited. This is particularly true in cases where longer periods are required to print complex or larger tissue constructs. Increasing the bioink's viscosity efficiently retards sedimentation but gives rise to cell membranolysis or functional disruption due to increased shear stress on the cells during the extrusion process. Inspired by the rainbow cocktail, we report the development of a multilayered modification strategy for gelatin methacryloyl (GelMA) bioink to manipulate multiple liquid interfaces, providing interfacial retention to retard cell sedimentation in the bioink reservoir. Indeed, the interfacial tension in our layer-by-layer bioink system, characterized by the pendant drop method, was found to be exponentially higher than the sedimental pull (Delta(Gravity-Buoyancy) = similar to 10(-9) N) of cells, indicating that the interfacial retention is crucial for preventing cell sedimentation across the adjacent layers. It was demonstrated that the encapsulated cells displayed better dispersibility in constructs bioprinted using the multilayered GelMA bioink system than that of pristine GelMA where the index of homogeneity of the cell distribution in the multilayered bioink was 4 times that of the latter.
机译：使用低粘度生物链挤出生物印刷的挑战之一是细胞的快速重力驱动沉降。在水凝胶生物链中的细胞，其特征在于具有低粘度的低粘度倾向于沉降到生物储存器的底部，因此，通过与沉积的非均匀细胞化结构相关联地阻碍它们的生物可燃性。在需要较长时段以打印复杂或更大的组织构建体的情况下，这尤其如此。提高生物链的粘度有效地延迟沉降，但由于在挤出过程中，由于细胞上的剪切应力增加，引起细胞膜或功能破坏。灵感来自彩虹鸡尾酒，我们报告了明胶甲基丙烯酰基（GELMA）生物链的多层改性策略，以操纵多种液体界面，提供界面保留以阻止生物储存器中的细胞沉降。实际上，发现由垂侧滴剂的逐层生物链接系统中的界面张力被认为是指数高于沉积的拉动（Delta（重力浮力）=类似于10（-9）n）的细胞，表明界面保留对于防止相邻层的细胞沉降至关重要。据证明，包封的细胞在使用多层凝胶生物链接系统的构建体中显示出比原始凝胶的结构更好的分散性，其中多层生物链中细胞分布的均匀性指数是后者的4倍。

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  • 来源
    《ACS applied materials & interfaces》 |2019年第34期|共11页
  • 作者

    Chen Nan; Zhu Kai; Zhang Yu Shrike; Yan Shiqiang; Pan Tianyi; Abudupataer Mieradilijiang; Yu Guodong; Alam Md. Fazle; Wang Li; Sun Xiaoning; Yu Yanlei; Wang Chunsheng; Zhang Weijia;

  • 作者单位

    Fudan Univ Dept Cardiac Surg Shanghai Med Coll Shanghai 200032 Peoples R China;

    Fudan Univ Dept Cardiac Surg Shanghai Med Coll Shanghai 200032 Peoples R China;

    Harvard Med Sch Brigham &

    Womens Hosp Dept Med Div Engn Med Boston MA 02139 USA;

    Fudan Univ Shanghai Med Coll Inst Biomed Sci Shanghai 200032 Peoples R China;

    Fudan Univ Shanghai Med Coll Inst Biomed Sci Shanghai 200032 Peoples R China;

    Fudan Univ Dept Cardiac Surg Shanghai Med Coll Shanghai 200032 Peoples R China;

    Fudan Univ State Key Lab Mol Engn Polymers Shanghai 200438 Peoples R China;

    Fudan Univ Shanghai Med Coll Inst Biomed Sci Shanghai 200032 Peoples R China;

    Fudan Univ Shanghai Med Coll Inst Biomed Sci Shanghai 200032 Peoples R China;

    Fudan Univ Dept Cardiac Surg Shanghai Med Coll Shanghai 200032 Peoples R China;

    Fudan Univ State Key Lab Mol Engn Polymers Shanghai 200438 Peoples R China;

    Fudan Univ Dept Cardiac Surg Shanghai Med Coll Shanghai 200032 Peoples R China;

    Fudan Univ Dept Cardiac Surg Shanghai Med Coll Shanghai 200032 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学工业;
  • 关键词

    3D bioprinting; bioink; interfacial retention; gelatin methacrylate; cell sedimentation;

    机译：3D BioPlinting;生物链;界面保留;明胶甲基丙烯酸酯;细胞沉降;

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