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首页> 外文期刊>ACS applied materials & interfaces >Fluorescent-Magnetic-Catalytic Nanospheres for Dual-Modality Detection of H9N2 Avian Influenza Virus
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Fluorescent-Magnetic-Catalytic Nanospheres for Dual-Modality Detection of H9N2 Avian Influenza Virus

机译：荧光磁性催化纳米H9N2禽流感病毒的双态态检测

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摘要

The outbreak of H9N2 avian influenza virus (H9N2 AIV) brings high mortality and huge economic losses every year. Sensitive and reliable detection methods are essential to timely diagnosis and treatment. Herein, a dual-modality immunoassay is proposed for H9N2 AIV detection by employing fluorescent-magnetic-catalytic nanospheres (FMCNs) as labels and alkaline phosphatase (ALP)-induced metallization as a signal amplification strategy. The excellent magnetic properties of FMCNs endow the assay a potential application in complex samples. Also, the excellent fluorescence properties of FMCNs enable fluorescence modality readout. The antibodies on the FMCN surface can achieve efficient capture and separation of targets. Amplified electrochemical modality readout can be obtained through ALP-catalyzed silver deposition. Dual-modality immunoassay combined the advantages of electrochemical assay with fluorescence assay and provides accurate detection results to meet different testing needs. With two quantitative analysis forms, H9N2 AIV can be detected by electrochemical signals with a quantitation range of 0.1 to 1000 ng/mL and a detection limit of 10 pg/mL. The linear range is 300 to 1000 ng/mL with a detection limit of 69.8 ng/mL by the fluorescence signal readout. Moreover, the specificity, anti-interference ability, accuracy, and diversity of the proposal have unlimited potential for early diagnosis of suspect infections.
机译：H9N2禽流感病毒（H9N2 AIV）的爆发每年带来高死亡率和巨大的经济损失。敏感和可靠的检测方法对于及时诊断和治疗至关重要。在此，提出了通过使用荧光磁性催化纳米体（FMCN）作为标记和碱性磷酸酶（ALP）诱导的金属化作为信号扩增策略来进行双式免疫测定检测。 FMCN的优异磁性赋予了复杂样品中的潜在应用。此外，FMCNS的优异荧光性能使荧光模态读出。 FMCN表面上的抗体可以实现有效的捕获和分离靶标。通过Alp催化的银沉积可以获得扩增的电化学模态读出。双态免疫测定结合了电化学测定与荧光测定的优点，提供了准确的检测结果，以满足不同的测试需求。通过两种定量分析形式，可以通过电化学信号检测H9N2 AIV，其定量范围为0.1至1000ng / ml，检测限为10 pg / ml。线性范围为300至1000ng / ml，荧光信号读出的检出限为69.8ng / ml。此外，提案的特异性，抗干扰能力，准确性和多样性具有无限诊断患病感染的无限潜力。

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  • 来源
    《ACS applied materials & interfaces》 |2019年第44期|共9页
  • 作者

    Xiaolun Peng; Guan Luo; Zhen Wu; Wei Wen; Xiuhua Zhang; Shengfu Wang;

  • 作者单位

    Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules College of Chemistry and Chemical Engineering Hubei University;

    Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules College of Chemistry and Chemical Engineering Hubei University;

    Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules College of Chemistry and Chemical Engineering Hubei University;

    Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules College of Chemistry and Chemical Engineering Hubei University;

    Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules College of Chemistry and Chemical Engineering Hubei University;

    Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules College of Chemistry and Chemical Engineering Hubei University;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学工业;
  • 关键词

    fluorescent-magnetic-catalytic nanospheres; dual-modality immunoassay; avian influenza virus; alkaline phosphatase-induced metallization; two quantitative analysis forms;

    机译：荧光 - 磁性催化纳米球;双式免疫测定;禽流感病毒;碱性磷酸酶诱导的金属化;两种定量分析形式;

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