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首页> 外文期刊>ACS applied materials & interfaces >Rapid and Robust Coating Method to Render Polydimethylsiloxane Surfaces Cell-Adhesive
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Rapid and Robust Coating Method to Render Polydimethylsiloxane Surfaces Cell-Adhesive

机译：快速耐用的涂层方法，以呈现聚二甲基硅氧烷表面细胞粘合剂

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摘要

Polydimethylsiloxane (PDMS) is a synthetic material with excellent properties for biomedical applications because of its easy fabrication method, high flexibility, permeability to oxygen, transparency, and potential to produce high-resolution structures in the case of lithography. However, PDMS needs to be modified to support homogeneous cell attachments and spreading. Even though many physical and chemical methods, like plasma treatment or extracellular matrix coatings, have been developed over the last decades to increase cell–surface interactions, these methods are still very time-consuming, often not efficient enough, complex, and can require several treatment steps. To overcome these issues, we present a novel, robust, and fast one-step PDMS coating method using engineered anchor peptides fused to the cell-adhesive peptide sequence (glycine-arginine-glycine-aspartate-serine, GRGDS). The anchor peptide attaches to the PDMS surface predominantly by hydrophobic interactions by simply dipping PDMS in a solution containing the anchor peptide, presenting the GRGDS sequence on the surface available for cell adhesion. The binding performance and kinetics of the anchor peptide to PDMS are characterized, and the coatings are optimized for efficient cell attachment of fibroblasts and endothelial cells. Additionally, the applicability is proven using PDMS-based directional nanotopographic gradients, showing a lower threshold of 5 μm wrinkles for fibroblast alignment.
机译：聚二甲基硅氧烷（PDMS）是一种合成材料，具有用于生物医学应用的优异性能，因为其易于制造方法，高柔韧性，氧气，透明性和在光刻中产生高分辨率结构的潜力。然而，需要修改PDM以支持均匀的细胞附着物和扩散。尽管在过去的几十年里已经发展了许多物理和化学方法，如血浆治疗或细胞外基质涂层，以增加细胞表面相互作用，但这些方法仍然非常耗时，往往不足，复杂，并且可能需要几个治疗步骤。为了克服这些问题，我们介绍了一种使用与细胞 - 粘合剂肽序列（甘氨酸 - 精氨酸 - 甘氨酸 - 天冬氨酸 - 丝氨酸，GRGDS）融合的工程化锚定肽的新型，稳健和快速的一步PDMS涂层方法。锚定肽通过在含有锚定肽的溶液中简单地浸渍PDMS，主要通过疏水相互作用，呈现在可用于细胞粘附的表面上的GRGDS序列。锚定肽与PDMS的结合性能和动力学的特征在于，并且涂层被优化以用于成纤维细胞和内皮细胞的有效细胞附着。另外，使用基于PDMS的定向纳米复印梯度证明适用性，显示用于成纤维细胞对准的5μm皱纹的较低阈值。

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