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首页> 外文期刊>ACS applied materials & interfaces >Antibody-Functional Microsphere-Integrated Filter Chip with Inertial Microflow for Size-Immune-Capturing and Digital Detection of Circulating Tumor Cells
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Antibody-Functional Microsphere-Integrated Filter Chip with Inertial Microflow for Size-Immune-Capturing and Digital Detection of Circulating Tumor Cells

机译：具有惯性微射线的抗体功能微球集成滤芯，用于尺寸免疫捕获和循环肿瘤细胞的数字检测

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摘要

Circulating tumor cells (CTCs) in blood is the direct cause of tumor metastasis. The isolation and detection of CTCs in the whole blood is very important and of clinical value in early diagnosis, postoperative review, and personalized treatment. It is difficult to separate all types of CTCs that efficiently rely on a single path due to cancer cell heterogenicity. Here, we designed a new kind of "filter chip" for the retention of CTCs with very high efficiency by integrating the effects of cell size and specific antigens on the surface of tumor cells. The filter chip consists of a semicircle arc and arrays and can separate large-scale CTC microspheres, which combined with CTCs automatically. We synthesized interfacial zinc oxide coating with nanostructure on the surface of the microsphere to increase the specific surface area to enhance the capturing efficiency of CTCs. Microspheres, trapped in the arrays, would entrap CTCs, too. The combination of the three kinds of strategies resulted in more than 90% capture efficiency of different tumor cell lines. Furthermore, it is easy to find and isolate the circulating tumor cells from the chip as tumor cells would be fixed inside the structure of a filter chip. To avoid the high background contamination when a few CTCs are surrounded by millions of nontarget cells, a digital detection method was applied to improve the detection sensitivity. The CTCs in the whole blood were specifically labeled by the antibody-DNA conjugates and detected via the DNA of the conjugates with a signal amplification. The strategy of the antibody-functional microsphere-integrated microchip for cell sorting and detection of CTCs may find broad implications that favor the fundamental cancer biology research, the precise diagnosis, and monitoring of cancer in the clinics.
机译：血液中循环肿瘤细胞（CTC）是肿瘤转移的直接原因。整个血液中CTC的分离和检测非常重要，早期诊断，术后审查和个性化治疗中的临床价值非常重要。难以将所有类型的CTC分离，这些CTCS由于癌细胞异质性而有效地依赖单个路径。在这里，我们设计了一种新的“滤器芯片”，通过将细胞尺寸和特异性抗原在肿瘤细胞表面上的效果与细胞尺寸和特异性抗原的影响相结合来保持CTC。过滤芯片由半圆电弧和阵列组成，可以将大型CTC微球分离，其自动与CTC组合。在微球表面上用纳米结构合成界面氧化锌涂层，以增加比表面积，以提高CTC的捕获效率。捕获在阵列中的微球也会捕获CTC。三种策略的组合导致不同肿瘤细胞系的90％以上的捕获效率。此外，随着肿瘤细胞固定在过滤器芯片的结构内，易于发现和将循环肿瘤细胞与芯片隔离。为了避免少数CTC包围数百万个不确定细胞时，避免高背景污染，应用了数字检测方法来提高检测灵敏度。全血中的CTC由抗体-DNA缀合物特异性标记，并通过缀合物的DNA与信号放大检测。用于细胞分选和CTC的细胞分选和检测的抗体功能性微球体综合微芯片的策略可能涉及临床基础癌症生物学研究，精确诊断和监测诊所的巨大意义。

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  • 来源
    《ACS applied materials & interfaces》 |2019年第33期|共10页
  • 作者

    Su Yi; Tian Qingchang; Pan Dingyi; Hui Lanlan; Chen Yanni; Zhang Qi; Tian Wei; Yu Jie; Hu Shen; Gao Yang; Qian Dahong; Xie Tian; Wang Ben;

  • 作者单位

    Zhejiang Univ Sch Med Affiliated Hosp 2 Canc Inst Key Lab Canc Prevent &

    Intervent China Hangzhou 310009 Zhejiang Peoples R China;

    Zhejiang Univ Sch Med Affiliated Hosp 2 Canc Inst Key Lab Canc Prevent &

    Intervent China Hangzhou 310009 Zhejiang Peoples R China;

    Zhejiang Univ Dept Engn Mech Key Lab Soft Machines &

    Smart Devices Zhejiang Pr Hangzhou 310027 Zhejiang Peoples R China;

    Zhejiang Univ Sch Med Affiliated Hosp 2 Canc Inst Key Lab Canc Prevent &

    Intervent China Hangzhou 310009 Zhejiang Peoples R China;

    Zhejiang Univ Sch Med Affiliated Hosp 2 Canc Inst Key Lab Canc Prevent &

    Intervent China Hangzhou 310009 Zhejiang Peoples R China;

    Zhejiang Univ Sch Med Affiliated Hosp 1 Dept Hepatobiliary &

    Pancreat Surg Hangzhou 310003 Zhejiang Peoples R China;

    Zhejiang Univ Sch Med Affiliated Hosp 2 Dept Surg Oncol Hangzhou 310009 Zhejiang Peoples R China;

    Hangzhou Watson Biotech Inc Hangzhou 310051 Zhejiang Peoples R China;

    Zhejiang Univ Sch Med Affiliated Hosp 2 Dept Obstet Hangzhou 310009 Zhejiang Peoples R China;

    Beihang Univ Sch Biol Sci &

    Med Engn Minist Educ Key Lab Biomech &

    Mechanobiol Beijing 100083 Peoples R China;

    Shanghai Jiao Tong Univ Sch Biomed Engn Shanghai 200030 Peoples R China;

    Hangzhou Normal Univ Coll Med Affiliated Hosp Dept Med Oncol Holist Integrat Oncol Inst Hangzhou 311100 Zhejiang Peoples R China;

    Zhejiang Univ Sch Med Affiliated Hosp 2 Canc Inst Key Lab Canc Prevent &

    Intervent China Hangzhou 310009 Zhejiang Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学工业;
  • 关键词

    circulating tumor cells; microfluidics; microspheres; antibody-DNA conjugate; liquid biopsy;

    机译：循环肿瘤细胞;微流体;微球;抗体-DNA缀合物;液体活组织检查;

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