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首页> 外文期刊>ACS applied materials & interfaces >Dopamine-Based Paper Analytical Device for Truly Equipment-Free and Naked-Eye Biosensing Based on the Target-Initiated Catalyzed Oxidation
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Dopamine-Based Paper Analytical Device for Truly Equipment-Free and Naked-Eye Biosensing Based on the Target-Initiated Catalyzed Oxidation

机译：基于多巴胺的纸张分析装置，用于基于目标引发的催化氧化的真正无裸眼生物溶解

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摘要

The development of low cost, portable, and disposable biosensors for equipment-free and naked-eye biosensing is in eager demand for their widespread application in biomedical field, but it is still a challenge. Herein, we propose a novel paper analytical device (PAD) for truly equipment-free and naked-eye biosensing using dopamine as the chromogenic agent based on target-initiated catalyzed oxidation reaction. The dopamine-functionalized PAD (DPAD) possesses a significant three-dimensional net structure, excellent hydrophilicity, and unique response toward G-quadruplex DNAs against other DNAs, benefiting the bio/chemo reaction occurrence to assay target bio-molecules. In light of the exceptional properties, the fabricated DPAD was applied in the analysis of Dam MTase through target-triggered exponential isothermal amplification. The recognition and methylation of H1 by Dam MTase contribute to formation of abundant hemin/G-quadruplexes, which catalyze oxidation of dopamine into dopachrome and reduce the dopamine amount on the DPAD surface. In comparison with the case in which Dam MTase is absent, an evident deep pink signal originating from dopachrome is observed directly by the naked eye and relied on Dam MTase concentrations. Therefore, truly equipment-free and naked-eye detection of Dam MTase is achieved with a detection limit of 1.46 U/mL. The fabricated DPAD not only achieves Dam MTase-visualized detection but also permits the accurate determination of other analytes by varying recognizable DNA's sequences, thus offering a universal biosensor and depicting significant potential for widespread applications in biomedical field.
机译：用于设备无需设备和裸眼生物传感器的低成本，便携和一次性生物传感器的开发是渴望其在生物医学领域的广泛应用中的需求，但仍然是一项挑战。这里，我们提出了一种新的纸张分析装置（垫），用于使用多巴胺作为基于靶引发的催化氧化反应的多巴胺作为发色剂的真正无裸眼生物溶解的新型纸张分析装置（垫）。多巴胺官能化垫（DPAD）具有显着的三维净结构，优异的亲水性和对其他DNA的G-Quadflex DNA的独特反应，使Bio / Chemo反应发生测定靶生物分子。鉴于特殊的性质，通过目标触发指数等温扩增在坝MTase的分析中应用了制造的DPAD。抑制H1的识别和甲基化有助于形成丰富的血红蛋白/ g-四链体，其将多巴胺氧化催化到多穴中并减少DPAD表面上的多巴胺量。与不存在坝MTase的情况相比，通过肉眼直接观察到源自多穴的明显的深粉红色信号，并依赖于坝MTase浓度。因此，通过1.46u / ml的检测限实现了真正的无坝MTase的无裸眼睛检测。制造的DPAD不仅达到坝MTase-可视化检测，而且还可以通过改变可识别的DNA的序列来精确测定其他分析物，从而提供通用的生物传感器并描绘了生物医学领域的广泛应用的显着潜力。

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