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首页> 外文期刊>ACS applied materials & interfaces >In Vitro Osteocompatibility and Enhanced Biocorrosion Resistance of Diammonium Hydrogen Phosphate-Pretreated/Poly(ether imide) Coatings on Magnesium for Orthopedic Application
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In Vitro Osteocompatibility and Enhanced Biocorrosion Resistance of Diammonium Hydrogen Phosphate-Pretreated/Poly(ether imide) Coatings on Magnesium for Orthopedic Application

机译：在骨科应用上的磷酸氢 - 预处理/聚（醚Imide）涂层的体外骨质组织和增强的生物腐蚀性抗性

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摘要

Magnesium, as a biodegradable metal, is a promising candidate for biomedical applications. To modify the degradation behavior of magnesium and improve its osteocompatibility, chemical conversion and spin coating methods were combined to develop a diammonium hydrogen phosphate-pretreated/poly(ether imide) (DAHP/PEI) co-coating system. The diammonium hydrogen phosphate pretreatment was employed to enhance the attachment between PEI coatings and the magnesium substrate; meanwhile, it could serve as another bioactive and anticorrosion layer when PEI coatings break down. Surface characterization, electrochemical tests, and short-term immersion tests in DMEM were performed to evaluate DAHP/PEI coatings. Electrochemical measurements showed that DAHP/PEI coatings significantly improved the corrosion resistance of pure magnesium. No obvious changes of the chemical compositions of DAHP/PEI coatings occurred after 72 h of immersion in DMEM. An in vitro cytocompatibility study confirmed that viability and LDH activity of human osteoblast-like cells on DAHP/PEI coatings showed higher values than those on the DAHP-pretreated layer and pure magnesium. The DAHP-pretreated layer could still enhance the ALP activity of MG-63 cells after the degradation of PEI in DAHP/PEI coatings. Besides that, the in vitro cellular response to the treated magnesium was investigated to gain knowledge on the differentiation and proliferation of human adipose-derived stem cells (hADSCs). Cell distribution and morphology were observed by fluorescence and SEM images, which demonstrated that DAHP/PEI coatings facilitated cell differentiation and proliferation. The high level of C-terminals of collagen type I production of hADSCs on DAHP/PEI coatings indicated the potential of the coating for promoting osteogenic differentiation. Positive results from long-term cytocompatibility and proliferation tests indicate that DAHP/PEI coatings can offer an excellent surface for hADSCs.
机译：镁作为可生物降解的金属，是生物医学应用的有希望的候选者。为了改变镁的降解行为，改善其疏松疏松骨质化，组合化学转化和旋涂方法，形成磷酸氢 - 预处理/聚（醚酰亚胺）（DAHP / PEI）共涂体系。采用磷酸二铵预处理来增强PEI涂层和镁基材之间的附着;同时，当PEI涂层分解时，它可以作为另一种生物活性和防腐层。进行表面表征，电化学试验和DMEM中的短期浸渍试验以评估DAHP / PEI涂层。电化学测量表明，DAHP / PEI涂层显着提高了纯镁的耐腐蚀性。在DMEM中72小时后，在72小时后发生了DAHP / PEI涂层的化学成分的明显变化。体外细胞膜相容性研究证实，DAHP / PEI涂层上人骨细胞样细胞的活力和LDH活性显示比DAHP预处理层和纯镁上更高的值。在DAHP / PEI涂层中PEI降解后，DAHP预处理层仍然可以增强MG-63细胞的ALP活性。除此之外，研究了对处理过的镁的体外细胞反应，以获得人脂肪衍生的干细胞（HADSCs）的分化和增殖。通过荧光和SEM图像观察细胞分布和形态，表明DAHP / PEI涂层促进细胞分化和增殖。 DAHP / PEI涂层上HADSCs的胶原型I型C末端的高水平C末端表明促进骨质发生分化的涂层的电位。长期细胞相容性和增殖试验的阳性结果表明，DAHP / PEI涂层可以为HADSCS提供优异的表面。

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