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首页> 外文期刊>ACS applied materials & interfaces >Gold Nanoparticle-Based Photoluminescent Nanoswitch Controlled by Host-Guest Recognition and Enzymatic Hydrolysis for Arginase Activity Assay
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Gold Nanoparticle-Based Photoluminescent Nanoswitch Controlled by Host-Guest Recognition and Enzymatic Hydrolysis for Arginase Activity Assay

机译：基于金纳米粒子的光致发光纳米开关，由宿主 - 访客识别和酶活性用于酶活性测定

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摘要

The development of simple yet powerful methods for monitoring enzyme activity is of great significance. Herein, a facile, convenient, cost-effective, and continuous fluorescent method for the detection of arginase and its inhibitor has been reported based on a host guest interaction-controlled and enzymatic hydrolysis-controlled luminescent nanoswitch. The fluorescence intensity of 6-aza-2-thioth-yrnine-stabilized gold nanoparticle (ATT-AuNP) is enhanced by L-arginine, owing to the formation of a supramolecular host guest assembly between the guanidine group of L-arginine and ATT molecules capped on the Au-NP surface. However, hydrolysis of L-arginine, catalyzed by arginase, leads to a decrease in the fluorescence intensity of L-arginine/ATT-AuNPs hybrids. Upon incorporation of the arginase inhibitor L-norvaline, the fluorescence of the ATT-AuNP-based detecting system is restored. The linear range of arginase activity determination is from 0.0625 to 1.15 U/mL and the limit of detection is 0.056 U/mL. The half-maximal inhibition value IC50 of L-norvaline is determined to be 5.6 mM. The practicability of this luminescent nanoswitch is validated by assaying the arginase activity in rat liver and monitoring the response of rat liver arginase to pharmacological agent. Compared to the existing fluorescent method of arginase activity assay, the approach demonstrated here does not involve any complicated technical manipulation, thereby greatly simplifying the detection steps. We propose that this AuNP-based luminescent nanoswitch would find wide applications in the field of life sciences and medicine.
机译：用于监测酶活性的简单且强大的方法的开发具有重要意义。本文，基于主体客体相互作用控制和酶水解控制的发光纳米开关，已经报道了用于检测氨基酶及其抑制剂的容易，方便，经济效率和连续的荧光法。 L-精氨酸增强了6-AZA-2-Thioth-yrnine-稳定的金纳米颗粒（ATT-AUNP）的荧光强度，由于L-精氨酸和ATT分子的胍基团之间的超分子主体访客组件形成盖上Au-np表面。然而，通过氨基酶催化的L-精氨酸的水解导致L-精氨酸/ att-AUNPS杂种的荧光强度降低。掺入氨基酶抑制剂L-诺韦伐，恢复基于ATT-AUNP的检测系统的荧光。精氨酸活性测定的线性范围为0.0625至1.15 U / mL，检测限为0.056 U / mL。 L-NORVALINE的半最大抑制值IC50确定为5.6mm。通过测定大鼠肝脏的氨基酶活性并监测大鼠肝脏氨基酶对药理剂的响应来验证该发光纳米开关的实用性。与现有的氨基酶活性测定的荧光法相比，这里证明的方法不涉及任何复杂的技术操作，从而大大简化了检测步骤。我们提出这种基于AUNP的发光纳米开关将在生命科学和医学领域找到广泛的应用。

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  • 作者

    Deng Hao-Hua; Shi Xiao-Qiong; Peng Hua-Ping; Zhuang Quan-Quan; Yang Yu; Liu Ai-Lin; Xia Xing-Hua; Chen Wei;

  • 作者单位

    Fujian Med Univ Dept Pharmaceut Anal Higher Educ Key Lab Nano Biomed Technol Fujian Pr Fuzhou 350004 Fujian Peoples R China;

    Fujian Med Univ Dept Pharmaceut Anal Higher Educ Key Lab Nano Biomed Technol Fujian Pr Fuzhou 350004 Fujian Peoples R China;

    Fujian Med Univ Dept Pharmaceut Anal Higher Educ Key Lab Nano Biomed Technol Fujian Pr Fuzhou 350004 Fujian Peoples R China;

    Fujian Med Univ Dept Pharmaceut Anal Higher Educ Key Lab Nano Biomed Technol Fujian Pr Fuzhou 350004 Fujian Peoples R China;

    Fujian Med Univ Dept Pharmaceut Anal Higher Educ Key Lab Nano Biomed Technol Fujian Pr Fuzhou 350004 Fujian Peoples R China;

    Fujian Med Univ Dept Pharmaceut Anal Higher Educ Key Lab Nano Biomed Technol Fujian Pr Fuzhou 350004 Fujian Peoples R China;

    Nanjing Univ Sch Chem &

    Chem Engn State Key Lab Analyt Chem Life Sci Nanjing 210093 Jiangsu Peoples R China;

    Fujian Med Univ Dept Pharmaceut Anal Higher Educ Key Lab Nano Biomed Technol Fujian Pr Fuzhou 350004 Fujian Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学工业;
  • 关键词

    gold nanoparticle; host guest recognition; photoluminescence; arginase; l-arginine;

    机译：金纳米粒子;寄主访客识别;光致发光;氨基酶;L-精氨酸;

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